We knew that inflammasome event induced by LPS and extracellular ATP could lead to the cell pyroptosis and death, but what about the effect of only ATP administration to the cell culture? Is it also toxic and induce cell apoptosis? Currently, I am doing an experiment regarding the inflammasome mechanism in cell culture. I observed that the only ATP triggers loss of cell adhesion and morphological changes. I am just wondered whether it was normal or not.
It may lead to activation of purinergic receptors on the cell surface which may results in formation of a large pores on cell membrane, leading to cell death.
Make sure you buffer your ATP well - it's very acidic when dissolved just in the water. Otherwise, low pH might have unintended consequences... ATP alone shouldn't cause much normally in my experience. Of course, there is always a chance that P2YRs stimulation might induce something, as Hani pointed out
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