When inducing pyroptosis, is it necessary to remove LPS and then add ATP?

08 May 2023 1 2K Report

In the experiment of inducing pyroptosis of THP1 cells, when adding ATP after pretreatment of cells with LPS, is it necessary to remove the LPS and then add the medium containing ATP, or add ATP directly to the medium containing LPS?

Julia A Guzova

you do not have to remove LPS to induce pyroptosis prior to adding ATP (or other pyroptosis activators like nigericin) - add ATP directly to LPS pretreated cells.

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