What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View

Why are we measuring gel content of NBR latex? Does gel content related to the amount of cross-linked in a polymer?

Hi good day to everyone! I am actually studying the gel content of synthetic latex. Refer to ASTM 3616, gel content is measure of insoluble rubbers in the chosen solvent. So, I just would like to...

07 February 2021 605 4 View

How to make ppb level concentration ?

Hi, I've N2 gas cylinder and 100 ppm NH3 gas cylinder. With these two cylinders, I'd like to make a ppb level (1- 100) concentration. Any idea how to do that?

01 February 2021 3,264 2 View

What can I do if DNA OD260/280 is either 1.6 or higher than 3?

26 January 2021 7,830 4 View

How do you multiple impute item scores and perform a linear regression on factor scores?

I am developing a questionnaire and first performing an exploratory factor analysis. After I have the final factor structure, I plan on regressing the factor scores on some demographic covariates....

18 January 2021 883 8 View

What will be the possible reasons to cause no bands on gel electrophoresis?

Hi, I am doing RNA extraction for my final year project. After extraction of RNA by using conventional method, I did quantification and qualification of RNA by using Nanodrop spec and gel...

11 January 2021 8,775 10 View

How to delete a copy of a paper, and how to delete a co-author's name?

I mistakenly uploaded a copy of the same paper and entered my alternative name. How to delete them out? (I couldn't navigate to the right buttons to do it. So from where to where is what I don't...

07 January 2021 7,694 6 View

What effect will different solvents have on the ATRP reaction?

I want to do an ATRP reaction, synthesize an ATRP initiator, use DMF as a solvent, and mix DMF, BIBB and TEA to turn red. Why does it turn red? Xylene has been used as a solvent before, and there...

04 January 2021 8,034 3 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Can anyone elaborate the method to measure arterial pulse wave velocity in research studies involving Sphygmocor??

Sphygmocor is the tonometry based instrument which is used to record generally radial pulse waves and then calculating various variables like Augmentation Index, SEVR etc. It is also used to...

02 February 2021 7,546 3 View

How can I carry out analysis of pulp and paper properties according to Tappi standard method in the Lab?

Properties such as relative humidity (T 402sp-03), brightness and yellowness (T-452 om-02), whiteness (T-560 pm-96), opacity and absorption coefficient (T-425 om-01), tensile, breaking length and...

24 January 2021 308 3 View

Cause of blisters in ALD layer at the edge of electrode?

I've encountered a defect during processing that I can't figure out, so I'm hoping I might find some ideas here. My mask design is a microelectrode array, with 60 microelectrodes with diameter...

19 January 2021 665 1 View

Cytokine levels in tears sample?

Hi dear, Do you have any idea of a commercial lab who measure the cytokines levels in tears by multiplex or flow cytometry? Thank you

18 January 2021 5,928 3 View

How to remove ORO (Oil red O) background precipitates?

Hi ORO experts, I'm struggling with ORO background precipitates in arterial tissues. The protocol I use works very well on human atherosclerotic plaques which are full of lipids and I don't...

13 January 2021 7,110 1 View

Has anyone seen this perivascular black stuff in rat brains?

This photo is from a reportedly healthy Long-Evans rat. Several of these animals have similar findings. No other sign of illness. Has anyone out there seen this?

19 November 2020 8,108 4 View

How to prevent hot crack/hot tear defect during the solidification of Cu5MCuC Inconel alloy ?

The Cu5MCuC Inconel casting produced by pouring into a sand mold seems to have developed several hot tears/hot cracks. The cracks opened up after heat treatment. How the tears/cracks could be...

01 November 2020 190 5 View

Dear brothers:It is real that the flecainida not tear apart the mother and to the fetus during his use in the flecainida in the fibrillation auricle?

08 September 2019 3,456 4 View

Has anyone had problems with brain tissue sticking during IHC?

Hi everyone, I've recently started having some issues with free floating brain tissue sticking to itself after coming out of primary and secondary antibody incubation. Prior to antibody...

07 August 2019 7,904 3 View