Manual homogenization using glass homogenizer takes lot of time, difficult to handle 50-100 tissue samples per day. Issue with mechanical homogenizer is it can destroy the fungal cells too.
In metal oxides how oxygen affect the electrical and magnetic properties? Also, how it impacts the EMI shielding performance. Please make discussion.
05 June 2024 483 0 View
I want to make reconfigurable RF and microwave filter, I need to connect low capacitance (minmum-0.048pF) varactor diode (MA46H120 Series). Can we use this varactor diode (MA46H120 Series) at 6...
21 May 2024 7,504 2 View
We have elastic scattering excitation function data in tabular form and want to obtain partial wave scattering phase shift data for each partial wave say l=0, 1, 2....whats the process to do so...
19 April 2024 2,225 1 View
I have used the DLS machine (Zetasizer Software) to measure the size of micelle prepared. For the first 2 runs the quality report shows "Good", while the 3rd measurement shows as "Too...
17 April 2024 4,491 3 View
I am working on identification of copy number in a recombinant Chinese hamster cell line by southern hybridization. I am having issues with the transfer of DNA onto positively charged nylon...
08 February 2024 2,647 8 View
We did reaction of alpha-bromo-gamma-butyrolactone with aq. ammonia solution at RT to get alpha-amino-gamma-butyrolactone. Reaction completed on TLC. Water distilled completely but unable to...
07 February 2024 5,766 0 View
I tried different protein assay kits (BSA, fluorometric peptide), but it didn't work.
25 January 2024 3,341 0 View
For example, Data obtained from a rheometer is as follows 1. Static yield stress - 1400 Pa, 2. Dynamic yield stress- 640 Pa, 3. Plastic viscosity-68 Pa-Sec, How are these values exactly...
24 January 2024 6,120 0 View
while working with low concentrated mammalian genomic DNA, can we increase the restriction digestion reaction volume to 200 ul to accommodate the required amount of mammalian genomic DNA for...
22 January 2024 6,952 12 View
how to select quality journal for publishing research work
11 December 2023 154 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...
03 August 2024 2,366 0 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View