In western blot, getting a thick cleaved band of gasD protein in the control sample. As samples are healthy subject then what will be the cause of getting cleaved band in untreated sample?
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I need a thesis on the above topic
28 July 2024 3,867 1 View
I didn't find any data source for continuous time series since 1950. NHB Residex and RBI give data from 2013 and 2010 onwards. Please guide. Thanks !!!
27 July 2024 6,271 5 View
The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.
23 July 2024 3,205 0 View
give suggestions
22 July 2024 5,664 2 View
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,284 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
What is the future scope of acoustic emission?
17 July 2024 1,510 1 View
I am conducting a study on Effectiveness of e-content on academic achievement of secondary school science student.
17 July 2024 5,633 4 View
Please suggest me, I want to present my research work.
13 July 2024 1,461 1 View
Hello everyone, I'm trying to extract proteins from J774A1 cells after treating them with 200ng/mL LPS for 4 hours followed by 5mM ATP treatment for another 4 hours to study inflammasome...
29 December 2022 378 0 View
Besides LDH, is there any method to quantitate pyroptosis (I am using THP-1). The results from LDH are not very consistant.
06 February 2022 3,253 2 View
So I am working with a drug that we know induces cell death in MDA-MB-231 cells. In other cell lines we have been able to identify that this drug induces ferroptosis. I have also done experiment's...
25 January 2021 3,928 3 View
Hi, I am working on the relationship between tissue damage repair and cell pyroptosis. The cell model I used were THP-1(human) and BMDM (mouse). This is the way I use to induce THP-1 cell...
13 October 2020 1,589 0 View
Hi all, I am currently running trials of IHC using fluorescent labeled secondary antibodies to detect pyroptosis markers on rabbit tissue. I am seeing fluorescence in tissue treated without the...
17 October 2019 2,820 3 View
09 October 2019 748 3 View
We knew that inflammasome event induced by LPS and extracellular ATP could lead to the cell pyroptosis and death, but what about the effect of only ATP administration to the cell culture? Is it...
19 September 2019 3,895 2 View
Hello all, I am designing an experiment to ectopically express caspase-1 and other components of the inflammasome to measure Il1b cleavage and release. However, I came upon this nice paper...
16 July 2019 8,712 1 View
How can I detect pyroptosis in human mo-DCs induced by a pathogen? Are there some comercial kit?
10 November 2018 5,284 0 View
There're some papers talking about inducing non-canonical pyroptosis using DOTAP and LPS. However, the detailed are not provided. For example, according to one paper, 1 μg of LPS-SM and 15 μl of...
31 December 2017 6,053 1 View