Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How to Implement Elastoplasticity in a PCL model in COMSOL Multiphysics?

I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...

23 February 2021 4,050 1 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...

09 February 2021 9,012 3 View

Why E1g mode (Molybdenum disulfide) is undetectable in Raman spectroscopy?

I synthesized 1T/2H hybrid phase MoS2 on a graphite foil surface by hydrothermal method, and then analyzed it by Raman spectroscopy. As a result, three new peaks correspond to the 1T phase were...

04 February 2021 6,580 2 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

How to estimate the extent to which the FE domain should be meshed with explicit elements in Explicit-Implicit Time integration procedure?

I am developing a MATLAB code for shock problem and want to divide the FE domain into explicit and implicit elements (region of domain at vicinity to shock is meshed into explicit elements and...

15 January 2021 1,968 2 View

Is it possible that insecticides from same group and same mode of action may not show cross resistance?

I could not find any cross resistance between two insecticides from OP group, is this possible?

14 January 2021 9,625 3 View

High throughput screen to identify drug induced cell death mechanism?

So I am working with a drug that we know induces cell death in MDA-MB-231 cells. In other cell lines we have been able to identify that this drug induces ferroptosis. I have also done experiment's...

25 January 2021 3,587 3 View

IHC- Getting fluorescence without the primary antibody?

Hi all, I am currently running trials of IHC using fluorescent labeled secondary antibodies to detect pyroptosis markers on rabbit tissue. I am seeing fluorescence in tissue treated without the...

17 October 2019 2,745 3 View

IHC- Getting fluorescence without the primary antibody?

Hi all, I am currently running trials of IHC using fluorescent labeled secondary antibodies to detect pyroptosis markers on rabbit tissue. I am seeing fluorescence in tissue treated without the...

09 October 2019 686 3 View

How to detect pyroptosis?

How can I detect pyroptosis in human mo-DCs induced by a pathogen? Are there some comercial kit?

10 November 2018 5,130 0 View

Does anyone know how to induce non-canonical pyroptosis?

31 December 2017 5,999 1 View

Can human IFI16 itself cleaved by caspase?

Hi All, The viral DNAs are sensed by IFI16 and induces caspase-1 activation and pyroptosis. Is it possible that caspase can target and cleave IFI16 and remove PYD domain?

10 November 2014 4,617 1 View

What is the diagnosis of pyroptosis? Is there a specific staining to diagnose pyroptotic cells?

Pyroptosis is a form of programmed cell death associated with antimicrobial responses during inflammation.

08 September 2014 1,041 4 View

How does IL-1b get secreted in in vitro inflammasome reconstitution systems?

Hi all, In vitro inflammasome reconstitution system is common in studies involving NLRP3, NLRP1 etc. Some examples of murine and human NLRP3 inflammasome reconstitution is linked...

01 January 1970 7,883 1 View

How does IL-1b get secreted in in vitro inflammasome reconstitution systems?

Hi all, In vitro inflammasome reconstitution system is common in studies involving NLRP3, NLRP1 etc. Some examples of murine and human NLRP3 inflammasome reconstitution is linked...

01 January 1970 6,881 1 View