Hello everyone,
We needed cardiac cells for a project and obtained H9C2 rat cardiovascular myoblast cells from another laboratory. The cells are at passage 15 and unfortunately have differentiated into cardiomyotubes before. Can this differentiation be simply reversed? Is there anything we can do?
Thank you very much.
Hello Gülşah Torkay ,
I have never worked with this particular type of myoblasts, but I do work with other myoblasts (C2C12, LHCNM2) and unfortunately, once the cells have differenciated into myotubes there is almost nothing you can do ... Dedifferenciation could be something to try if you have the time and energy, and I think some protocols exist but I am not at all familiar with them, and I don't know if myotubes are an easy target (if a target at all).
In my opinion, the best (and easiest) thing to do is to ask for new cells, if you can. The "high" passage doesn't mean they will have differenciated, but as each passage is a form of stress for the cells, and includes the risk that the cells reach more than the adequate confluence, the risk of receiving myotubes is higher : I would ask if possible for a lower passage.
Hope my answer helps a bit ! Good luck for your experiments !
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