How to adjust the seeding density of calculated cells suspension in 6 well plate for western Blot and Clonogenic assay?

06 October 2023 1 7K Report

Hello,

Please guide me in the followings,

I have an issue with cell seeding. When I made the cell suspension, and adding it into wells one by one, (shaking after every two/three wells), still the last two wells ended up with the high number of cells. How can I adjust the accurate number of cells in all wells please?

And related to the clonogenic assay, do i need to add the treatment each time when I replenish the medium.

Profound Regards!!!!

Aroona Razzaq

Alex Lyakhovich

(I) you may want to use multichannel pipettes and V-shaped bottom tray; (ii) single 3d-treatment is enough.

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