in my experiment, I would like to consult whether there is an internal reference protein that is specifically expressed in adopocytes(similar to gapdh, but only expressed in adipocytes.Used to distinguish adipocytes in adipose tissue)
Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...
01 March 2021 2,957 8 View
18 February 2021 9,188 3 View
There are two plates with horizontal center crack, one with crack length of a with the other with a+da. The bottoms are fixed while the tops are under the same displacement load. For intuition,...
11 February 2021 9,543 2 View
Hello Dear Researchers, I am confused about the Difference between Second-harmonic generation and Two-photon absorption? Can anyone describe the two phenomena briefly in the physical picture? What...
24 January 2021 9,912 5 View
I want to find a GPC available solvent to dissolve both lipid (DPPC, 2 mM) and salt (NaCl, 100 mM).
14 January 2021 3,805 2 View
I just developed a new theory on electromagnetic and plan to publish in a Journal. The findings are purely through strict mathematical derivation without any disputable assumptions and also...
13 January 2021 1,558 5 View
Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...
13 January 2021 8,336 4 View
I detect the plasma AngII concentration by AngII ELISA kit (ENZO), the results show that plasma AngII decrease in AngII infusion mice. I check with the kit and find that the kit could recognize...
31 December 2020 8,838 3 View
Hi, I use YASARA (version 17.8.15) on windows 10 and recently I am trying to install FoldX plugin on YASARA. Here is what I did: 1. Download YASARA plugin for windows (and the executable and...
24 December 2020 536 3 View
I am learning the knowledge about barge pier collision these days, and have a problem that the impact force of barge pier collision suddenly increase to the peak value in ls-dyna. But the...
05 December 2020 9,978 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...
24 February 2021 9,210 1 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View
We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...
22 February 2021 2,072 3 View
22 February 2021 9,932 11 View