Cell Culture Techniques

Cells in culture or in vitro are a useful model for studying the activity of cells in the whole organism or in vivo. Ten years ago or so cell... | Contact experts in Cell Culture Techniques to get answers

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Questions related to Cell Culture Techniques

Can BIRC5 be downrgulated in Lung Cancer?

I have found a significant gene (BIRC5) between lung cancer tumor and adjacent normal samples. In the literature, it is mentioned that BIRC5 (an anti-apopotic gene) is generally upregulatd is most...

09 December 2022 2,393 1 View

Can we calculate the theorical maximum adsorption capacity of an adsorbent before doing the experiment?

Hello dear researchers, If we have a known adsorbent and a known adsorbate, and each single entity of the adsorbent is supposed to be an active site of the adsorption. The molar mass of the...

09 December 2022 8,790 3 View

Can anybody please suggest which cell line could be the best to generate tumors in BALB/c mice?

I need to make a syngeneic mouse model of HER2+ breast cancer in BALB/c mice. Please share your opinion about using NF639.

06 December 2022 2,648 0 View

MSC in PBS for flow cytometry?

How to prepare cells derived from mesenchymal stem cell for flow cytometry? (1) Cells in PBS and stored in -80? (2) Cells in sterile saline and stored in -80? (3) Cells in PBS and stored in 4...

06 December 2022 339 0 View

Is there alkaline soil and alkali soil same?

According to some literature alkali and alkaline soil are same, and some shows those are different. so which one should be correct?

05 December 2022 7,665 3 View

How to dilute a working solution ?

Hi all, bit confused so I need a bit of explanation. How do I dilute a solution, lest say I have a working solution of 200 uM, and I wish to make it to lets say 20 uM. 1M =1000mM=1000000uM...

04 December 2022 5,626 0 View

Injections of QC standard yield increasing areas in negative ionization mode (LC/MS/MS)?

Hi all! I am using a Waters-TQS micro (LC/MS/MS) in negative ionization mode (MRM) to analyse a few compounds. When doing QC injections (i.e., repeated injections from same vial of standards at a...

01 December 2022 8,162 4 View

Does L wave occur in HFpEF mouse model?

Hi guys, Does anyone do echo for the HFpEF mouse model? I'm doing the echo for the HFpEF mice models which are fed with HFD and L-NAME. Those mice are under anesthesia for echo. And it's...

19 November 2022 7,884 0 View

What are some MUC1 negative pancreatic cell lines?

Hey, I'm looking for pancreatic cell lines don't express MUC1 or with knockouted MUC1 gene! Any suggestions and will be highly appreciated.

19 November 2022 8,136 0 View

Why HEK-blue hTLR4 cells are not used for TNF-a analysis?

Usually HEK-blue hTLR4 cells are used for NF-kB activity. But for cytokine (TNF-a, IL-6) analysis, people used macrophage cells (Raw 264.7 or PMA-differentiated THP-1). IS HEK-blue hTLR4 cells...

18 November 2022 4,235 1 View

How to control the endophytic fungi during plant tissue culture ?

I am working on micropropagation techniques in wood tree plants, but while doing the endophytic fungi shows dominance, can anyone suggest how to inhibit the endophytic fungi?

16 November 2022 2,448 6 View

Can size exclusion chromatography used for nanoparticles?

I want to use size exclusion chromatography for drug-encapsulated PLGA nanoparticles. I am going to use Sephadex. but I couldn't find any suitable procedure for nanoparticle separation using SEC....

15 November 2022 1,347 2 View

Can tumor cells derived from BALB/c mouse be applied in C57BL/6 mouse?

Hi guys, I'm working on mouse tumor models and I planned to inject tumor cells into C57BL/6 mice. Recently I found that tumor cells are derived from BALB/c mice. I also read some papers and...

15 November 2022 7,303 0 View

Why large fluctuations in the LDH determination?

I have large fluctuations in the individual values in the LDH determination (Kit Promega: CytoTox96 Non radioactive Cytotoxicity Assay) of primary neuron cultures. Anyone know if centrifuging the...

14 November 2022 692 0 View

What is the first mention of pharmaceutical salt of drug?

Hello! What are the oldest literary sources you know that would mention pharmaceutical salts? I am aware of the first cocrystal [benzoquinone + hydroquinone] (1:1) investigated in 1844, but I...

09 November 2022 3,562 7 View

How to calculate the dose of glucose for ipGTT in diet-induced mice?

Hi guys, I'm working on a mouse model (using C57BL/6J) that needs high-fat diet (HFD). Recently I plan to perform ipGTT, just testing blood glucose. My colleague thought 1g of glucose/kg body mass...

09 November 2022 3,610 7 View

Problem with MST (microscale thermophoresis) capillaries and can you reuse them?

Hi Nanotemper MST (microscale thermophoresis) users, have you noticed during the last year that your MST experiment stopped working with new batches of Nanotemper capillaries? We have been doing...

08 November 2022 1,035 0 View

Western Blot for Phospho eEF2?

Hello, I am getting lots of unspecific bands when I do western blot for the phospho eEF2, (when I do it for the total eEF2, i get only one clear band) can someone suggest a specific technique for...

04 November 2022 8,513 2 View

How to preserve fruiting body of Macrofungi?

Often after collection of Fruiting bodies of Macrofungi, they began to decompose almost after couple of hours which make it extremely difficult to carry out further works such as spore print,...

02 November 2022 5,285 2 View

MTT ASSAY I have a question regarding MTT Assay. I got a I gram and I want to dilute it with ethanol instead of PBS. Should I use 20mg/ml dilution?

I have a question regarding MTT Assay. I got a I gram and I want to dilute it with ethanol instead of PBS. Should I use 20mg/ml dilution?

29 October 2022 5,578 3 View

What is the Physiological salt concentration of the cell also inside nucleus?

I am actually performing an Md Simulation study of the protein+DNA complex and using Amber99 ff. but I am confused about what concentration of salt to take for the simulation.

25 October 2022 4,511 1 View

How can I start to write a review paper?

I am a newcomer in researching. I want to start this by write a review paper in anyone of the topics including renewable energy, automation or automobile sectors. How can I start this? I'll be...

21 October 2022 1,505 6 View

How to produce a triphosphate (3p) motif by in vitro RNA transcription?

I want to perform an in vitro RNA transcription to obtain an RNA strand with a 3p (Triphosphate) motif added at the 5' end. I have found some protocols but most only use a DNA template and the...

12 October 2022 5,493 1 View

How to increase glycolysis/glycolytic capacity in vitro while using Seahorse XF?

Dear all I am performing the mitostress seahorse assay in aortic endothelial cells. I am looking for a good positive control that would increase the glycolysis/glycolytic capacity when I treat the...

12 October 2022 4,316 1 View

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