Cell Culture Techniques

Cells in culture or in vitro are a useful model for studying the activity of cells in the whole organism or in vivo. Ten years ago or so cell... | Contact experts in Cell Culture Techniques to get answers

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Questions related to Cell Culture Techniques

I'm interested in this research; Can anyone provide the full text of this?

I'm interested in this research; Can anyone provide the full text of this? Irisin deficiency exacerbates diet-induced insulin resistance and cardiac dysfunction in type II diabetes in mice. DOI:...

26 October 2023 7,062 0 View

How to analyze sequencing data in a very large fastq file?

Hello, I'm trying to figure out how to analyze these fastq files. We received 2 files, one labeled R1 and the other R2. Its raw data and there seems to be some barcodes within the file as well....

26 October 2023 3,604 3 View

How can I ensure the purity of my DNA for PCR?

I'm facing challenges with the purity of plasmid DNA extracted from Trypanosoma brucei for PCR applications. Despite using a commercial DNA extraction kit, I suspect the presence of contaminants...

21 October 2023 801 3 View

How to collect samples of chickpea roots from field to avoid protein and metabolite change?

Greetings, I am working on proteo-metabolomics response of chickpea roots. I will do field experiment. I am confused about sampling of chickpea roots and transportation to laboratory, without...

16 October 2023 6,791 0 View

How to categorize mycorrhiza?

Does concept of mycorrhizas include arbuscular mycorrhizas or both are the same? I want to find a clear classification of arbuscular mycorrhizas/endomycorrhizas/ectomycorrhizas/orchid...

07 October 2023 7,297 2 View

What should be observed in the ID/IG ratio of rGO?

I have prepared the reduced graphene oxide using Ethanol. I have change the reduction timing and taking it in a several parts. What kind of pattern will be observed in the RAMAN spectra and what...

05 October 2023 9,804 0 View

Root-AMF colonization analysis non-destructive method?

Is there any non-destructive method (not to harvest the root) to quantify AMF colonization with plant roots in pot experiments or rhizotrons?

03 October 2023 9,418 0 View

How solubilize oleic acid to cell culture?

Hello I have been trying to solubilize oleic acid to add it in cell culture, as done in this article Insulin prevents fatty acid induced increase of adipocyte size "Oleic acid was prepared as...

03 October 2023 3,073 0 View

What is the relation of Vertical Development to public access to services?

how does Vertical Development relates to public access to services in a city traffic and service flow?

29 September 2023 4,221 0 View

How and When I select Cleaning pot and cleaning Time ?

When I use my Instrument 757 VA to run cyclic voltammetry. I see in the program Conditioning cycles and Pretreatment, and in the Pretreatment I found Cleaning potential and cleaning time, I make...

26 September 2023 3,456 0 View

How to calculate the CO2 conversion rate or percentage in photocatalytic reaction?

I am working on CO2 reduction by photocatalytic approach. The product is methanol, and the calculated methanol production rate is in terms of umol/g.h. I want to know how much CO2 converts to form...

23 September 2023 3,319 2 View

How to use Augmented design for two factors experiment ?

I want to test 33 genotypes of a crop under 4 drought levels, the number of check varieties in my experiment will be 3 in addition to the 33 testing genotypes. That is the total number of my seed...

21 September 2023 1,186 4 View

How % removal and adsorption capacity are related?

Hi I have done the removal of heavy metals from the aqueous solutions through batch experiments. I observed that with increasing the concentration adsorbent (g) the % removal of heavy metal has...

18 September 2023 560 17 View

Is it possible that a protein Fasta file contains its anti-body also?

I have a protein sequence file, and I noticed that there is a sequence in its Fasta file that is related to one of its antibodies. I want to do a docking between the antibody and the protein. Does...

16 September 2023 9,312 16 View

How to study elevation's impact on soil carbon while ignoring depth with data from two depths?

This question seeks guidance on conducting an analysis that examines the primary influence of elevation on soil carbon content while explicitly excluding the impact of soil depth as a variable of...

02 September 2023 3,875 6 View

Has anyone heard of conjugating BioId to a lipid (LPS)?

I know BioID typically has to be spliced into a gene of interest. But my project is trying to identify host cell proteins interacting with LPS. I am having trouble finding methods for doing a wide...

01 September 2023 5,723 0 View

Are there any chemical agents that can stop phase separation of peptide?

are there any chemical agents that can stop phase separation of peptide?

15 August 2023 9,721 1 View

How to improve yield and quality of RNA isolated from urinary exosomes for microRNA profiling ?

I was isolating the exosomes from 40 mL of urine sample by ultracentrifugation method and further try to isolate total RNA from it for NGS. I was able to isolate total RNA conc. around...

04 August 2023 8,761 2 View

Why does oral cancer not have a screening test in addition to the oral clinical examination in order to detect it early?

Oral cancer has a high morbidity and mortality rate and clinical examination has resulted in late diagnosis. What reason has led to a delay in the implementation of a complementary screening test...

03 August 2023 7,056 4 View

I am working on endophytic fungi, which classification system should I fallow ?

I am working diversity of endophytic fungi and I have followed the Hawkworth classification of fungi for my research work and already submitted my research data for publication but recently I...

31 July 2023 363 2 View

How can I convert .cdf mass spectrometry files to mzML?

I can't analyse my mass spectrometric data (from Hiden Analalytical MASSoft) because it only saves in .cdf or .csv files. I have tried MSConvert but it doesn't seem to support .cdf files. Does...

28 July 2023 8,841 0 View

Evaluation of antioxydant activity?

I evaluated the antioxydant of ethanolic extract by DPPH. I chose a concentration of 1000 ug/ml and then 2000 ug/ml very high concentractions but no yellow color appeared when adding DPPH. Knowing...

26 July 2023 9,856 6 View

Analyze glass fibers length by ImageJ?

Do you guys know how to precisely analyze the length of glass fibers by ImageJ? I am trying to analyze the glass fibers image that was taken by microscopy to get the length of fibers and their...

26 July 2023 9,171 0 View

Artificial Intelligence which is part of emerging technologies is currently dominating the world. Do you think it will lead to Unemployment or not?

The application of Artificial Intelligence in many sectors could have many positive impacts. However, other researchers are predicting the possibility of it, making many unemployed globally, as...

24 July 2023 7,298 4 View

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