Cell Culture Techniques

Cells in culture or in vitro are a useful model for studying the activity of cells in the whole organism or in vivo. Ten years ago or so cell... | Contact experts in Cell Culture Techniques to get answers

2,495 views 4,554 posts

Questions related to Cell Culture Techniques

Can we grow corn in desert area?

Calciorthid type of soil is found in this area. The average temperature is 32 degree Celsius.

22 May 2023 3,101 3 View

Can biomedicine be regarded, in this era, as an alternative healing idea/practice?

With the consideration of biomedicine as a "mainstream" health care approach, people regard various traditional and indigenous healing ideas and practices as alternative healing. When we look at...

18 May 2023 2,652 4 View

The eGFP mainly localized in the cytoplasm in HeLa cells after 24h transfection?

The eGFP I used is the one prone to be a weak dimer. Recently, when I transfected it into HeLa cells using Lipofectamine 2000 for 24h, I noticed that it only localized in the cytoplasm (observing...

17 May 2023 7,761 1 View

I want to do SEM analysis for Alginate hydrogel samples, how long do I need to lyophilisation to freeze drying the hydrogel?

Alginate hydrogel =1 mL

17 May 2023 434 2 View

Lab Inventory software?

Hello Please recommend me a lab inventory software Thanks

12 May 2023 9,698 4 View

Any suggestions for non-invasive small animal imaging equipment?

Dear all, Is there any equipment that can track the drug distribution in the mouse? We want to see if the drug or small molecules labeled with a fluorescent dye are enriched in the targeted...

11 May 2023 8,032 0 View

Does EdU interfere with ATAC-Seq?

Hello all, I am preparing an experiment to evaluate chromatin accessibility (ATAC-SeqAssay for Transposase-Accessible Chromatin using sequencing) in a mouse model. Since the number of cells needed...

08 May 2023 994 0 View

Can we store IPTG induced culture broth at 4C overnight?

We are inducing a periplasmic protein using 1mM IPTG at 37 degrees for 3 hours. We cannot centrifuge and remove supernatant the same day. So can we store the broth containing Bacterial cells and...

07 May 2023 7,729 4 View

Which media and FBS are suitable for LLC1?

Hi, I'm working on lung cancer cells, LLC1 (CRL-1642, ATCC) but I'm not familiar with it. which media and what kind of FBS are good for LLC1? or are there anything important I need to know? Many...

05 May 2023 1,108 3 View

Do these human anti-CD3 antibodies (clone HIT3a and OKT3) have the same binding epitope and compete with each other?

I stimulated human PBMCs with soluble anti-CD3 (clone HIT3a) and I wanted to assess these cells by flow using a T cell panel, but I am not sure whether the flow antibody I chose for CD3 (clone...

04 May 2023 3,724 0 View

How to determine the sampling sites to test for oil-degrading fungi?

Greetings everyone! I am a Biology undergraduate student and I am doing my thesis on the bioremediation capabilities of fungi in the recent Oriental Mindoro, Philippines oil spill. With that I...

26 April 2023 8,638 2 View

Endless PCR contamination using ITS2 for Pocillopora.sp symbiodiniaceae analysis ?

Hello everyone, for a couple of weeks now i try to realize a PCR amplification of DNA extract from coral of the species Pocillopora.sp using ITS2 primer (internal transcribed spacer region 2) for...

13 April 2023 4,827 5 View

What makes microbes that live in these extreme environments structurally different than those that do not&microbial organismscan survive extreme heat?

What makes the microbes that live in these extreme environments structurally different than those that do not and microbial organisms can survive extreme heat?

13 April 2023 2,373 3 View

What's the role of L-NAME in cancer?

Hi there, I used L-NAME to treat cancer cells. Then I did LDH and MTT, according to these results, MTT showed the cell viability was above 100% compared with control, and LDH showed cytotoxicity...

31 March 2023 7,489 3 View

How to purify cytokines from mouse ear sample?

Is there anybody out there who has experience how to isolate and purify common inflammatory cytokines (TNFa, IL-1b, IL-6 etc.) from inflamed mouse ear tissue? We usually do it with blender using...

30 March 2023 4,764 1 View

Is evolutionary biology an important class to study if you plan to go into medicine?

I'm currently writing a paper comparing medical students' impression of evolutionary biology prior to and after my country's adoption of a new K-12 curriculum that includes an explicit class...

29 March 2023 4,570 6 View

Cryosectioning Brain Tissue- Issues of OCT separating from tissue?

Hi, Im having a hard time getting good sectioning on my PFA fixed tissue. No matter how I try to cut my sections, brush method or anti roll plate, my tissue is coming apart from the OCT which...

28 March 2023 9,820 2 View

What are the most effective and cheaper ways to measure the ectomycorrhizal colonization percentage on roots?

Dear all, What are the best methodologies to find out the ectomycorrhizal colonization on roots? I am looking for a publishable methodology to have a tuber sp. percentages of my seedlings.

26 March 2023 6,141 1 View

How to handle two differing Position Weight Matrices of a DNA cognate site for a single Transcription Factor?

While handling a database, I have a single transcription factor sequence, but the database gives multiple Position Weight Matrices (PWMs) for the DNA site said TF binds. This is because the...

25 March 2023 6,556 2 View

The difference between fermentor/bioreactor and magnetic mixer system?

Can a fermentor be used as a magnetic mixer system, or are these two systems fundamentally different despite sharing some common parameters?

21 March 2023 3,102 2 View

ATAC-seq, Tn5, kits, protcol recommendations?

Hello fellow scientists. I am planning to do an ATAC-seq run in the near future and have a few questions and concerns. I have acquired the protocol used by a previous member of the lab I am in,...

16 March 2023 4,902 0 View

How to analysis the Indicator Species Analysis (ISA) in multiple year?

I want to analysis the Indicator Species Analysis (ISA) in microbial community based on eDNA. Do you have any suggestions?

13 March 2023 748 2 View

I want to seed 10000 cells per well in 96 well plate. will the cell concentration change or remain same if I add 200 ul as against 100ul??

I want to seed 10000 cells per well in 96 well plate. generally we add 100ul cell suspension. If we add 200ul cell suspension will it become more than 10000 cells

12 March 2023 3,483 0 View

Will the choice of certain mobile phase affect the ability to separate components of mixture?

I performed a chemical reaction, and I went to make TLC for my product. At first, I used ethyl acetate: hexane with different ratios, but no product was identified. However, when I made TLC using...

12 March 2023 1,067 1 View

Badges
Science method