MRS broth containing ingredients like ammonium citrate inhibit many microorganisms including gram negative bacteria, if i collect supernatant from mrs broth lactobacillus culture and use the supernatant against test strain by well diffusion method how can i know whether the zone of inhibition is given by supernatant or mrs broth itself against test strain?
This is depending on the type of test strain used. Fill one well with MRS broth and the other with suernstsnt of Lactobacillus strain
Note the result:
Borth are negrtive (no ininbition compounds)
Borth are positive (
inhibition may attributes to MRS)
The last
One positive (sample)and the other is negative (broth)
If the supernatant of lactobacillus gave positive result while MRS gave negative
The inibition zone attributes to the lactobacillus strain
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