I need examples of asceptic techniques that are used in plant tissue culture.
The skills needed for plant tissue culture involve learning several lab techniques. I do not know what experience you have, but I suppose you could say that there are 5 phases that you need to know about to be successful. Aseptic techniques are essential to culture plant tissues free of microorganisms.
1. Preparation of solid (agar) or liquid medium. You need suitable nutrients and growth promoting substances and the medium needs to be filtered-sterilized or autoclaved and in sterile plastic or glass containers.
2. You need a clean-air hood running. Wipe the inside with alcohol.
3. Select your plant and in the hood you surface sterilize the plant pieces with a suitable chemical (usually sodium hypochlorite solution). Then wash with autoclaved water in the hood.
4. Cut out inner plant tissues with sterile (autoclaved) dissection instruments or a cork-borer. Use alcohol and a flame to keep the instruments sterile while you are doing this.
5. Inoculate the medium with plant pieces having discarded any parts that were exposed to the chemical used for surface sterilization. Leave in petri dishes or other sterile plastic ware... probably with lights on. In time your plant parts should grow if you used a correct medium.
Good luck.
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