How to prepare cells derived from mesenchymal stem cell for flow cytometry?

(1) Cells in PBS and stored in -80?

(2) Cells in sterile saline and stored in -80?

(3) Cells in PBS and stored in 4 degree?

(4) Cells in sterile saline and in 4 degree?

(5) Freshly harvested cells kept in either PBS or sterile saline?