Cell Culture Techniques

Cells in culture or in vitro are a useful model for studying the activity of cells in the whole organism or in vivo. Ten years ago or so cell... | Contact experts in Cell Culture Techniques to get answers

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Questions related to Cell Culture Techniques

How to prove the association between a gene and Chromatin accessibility?

I want to use TUNEL staining to prove the association between a gene and Chromatin accessibility. However, I cannot control the experiment volume. What are the other ways to prove the association...

16 December 2021 6,575 1 View

How to find particle size distribution from image of Overlapping, non-spherical, inequally bright particles?

Overlapping particles edge' cannot be detected with most image analysis software. Thresholding distorts data of particle size when particles are unequally bright. Non-spherical-shaped particles...

14 December 2021 3,873 4 View

Can tebuconazole be autoclaved?

Hello, I am going to prepare a medium with tebuconazole? It is my first time to work on this fungicide. I want to know whether tebuconazole is thermalstable and can be autoclaved? Best wishes Zha

10 December 2021 1,170 7 View

Can anyone help me out to determine the purity of exopolysaccharides?

I am working on the bacterial exopolysaccharides, I need to check purity of the polysaccharide. Can anybody tell me what are the methods have been used for the above?

03 December 2021 9,720 2 View

Does anyone have any ideas about changing my western blot method to avoid multiple bindings?

Hello! After harvesting my cells, I resuspended them in RIPA buffer and stored them at -80°C. After determining the protein, I usually load 10-25 µg onto a 4-15% SDS gel. The protein on the gel...

01 December 2021 5,645 3 View

How Long does it Take for Tamoxifen to activate Cre in a Cre-ERT system?

1. How Long does it Take for Tamoxifen to activate Cre and knock out the gene of interest 2. What would the dosing regimen be for rats/mice? I am designing an experiment where I will be knocking...

30 November 2021 8,634 3 View

Can I autoclave trimethylamine N-oxide or filter sterilization is safer option?

I have to use TMAO as a supplement in my expression media to check if it affects the protein expression in any way. So for that, can I autoclave it or filter sterilize TMAO?

23 November 2021 4,706 0 View

3T3 Cells stop growing after a few passages?

I've had a few attempts in culturing 3T3 cells in my lab. We use DMEM supplemented with 10% FBS for them. From reading several articles, it's been said that 3T3 cells are relatively...

17 November 2021 998 0 View

How to remove exopolysaccharides from bacteria without killing them?

Hello, I am looking for a way to non-specifically remove exopolysaccharides (EPS) from bacteria while keeping the bacteria viable. I am working on a project where I separate cells from an...

12 November 2021 5,092 1 View

Suspension cells growing as adherent???

I'm working with BJAB lymphocytic cell line, culturing them with RPMI media (with L-glutamine) + 10%FBS + 1% antibiotics. After two days of changing media by centrifugation, they started to adhere...

09 November 2021 1,039 3 View

Culture medium other than RPMI for LCL?

Dear all, Has anyone ever used another medium than the "standard" RPMI + glutamine + FBS for the culture of EBV-transfected lymphoblastoid cell lines? RPMI would potentially interfere with our...

05 November 2021 467 2 View

Primary Hepatic Stellate cell culture contamination after serum starvation?

Hi i have been continuous culturing primary human hepatic stellate cells from donors for research. however, we started to encounter contamination after we perform a serum starvation on the cells...

30 October 2021 516 1 View

Interference of phenol - sulfuric acid assay?

Hi everyone. I am measuring carbohydrate content of some microalgae. While I was making the standard curve, I noticed that the cultural media (F2 medium and ESAW medium) can also react to phenol...

27 October 2021 4,705 3 View

How can I remove sodium salt from drugs ?

Hello, I want to discard sodium salt from drug (Nafcillin) ? We want to Nafcillin without salt, insoluble form in water ? So, is there any technic that we can apply ?

26 October 2021 5,983 0 View

Use of the medium in the formation of a hydrogen peroxide-induced senescence model in human fibroblast cells?

Which medium should I use for this experiment? it should contain no phenol red.

22 October 2021 5,515 3 View

How is the IC50 calculation in graphpad?

When ı want to calculate the ıc50 value of my drug in graphpad. It is said that ı must write 1 instead of concentration zero because of logarithm. But ı am confused that what will ı do if use 1...

21 October 2021 7,595 4 View

How can I prepare Zinc Chloride (ZnCl2) solution of density 1.5 g/cm3 ?

For microplastics Analysis

18 October 2021 7,837 4 View

How to dissolve rotenone in DMEM????

Hi guys,I am very confusing that how can I dissolve rotenone in DMEM? I am doing cell experiments. when I dissolve rotenone in DMEM + serum, the rotenone can completely dissolve (no small pellet...

11 October 2021 6,541 1 View

does shifting of hela cells from EMEM to DMEM affect its growth or any other parameter?

If I have maintained cell line EMEM and later I have shifted from Emem to DMEM is it affects the growth of any kind of Parameters sooner or later ?

07 October 2021 677 3 View

How to prepare 1000microg/ml glucose solution?

I need to prepare 1000microg/ml and I could not figure out the amount of glucose which I have to weight.

04 October 2021 1,855 6 View

Vitamin E and vitamin C concentrations as antioxidants in cell culture?

I want to test antioxidant activity of a compound in neuronal cell culture and compare it with vitamin E (or Trolox) and vitamin C effects. Which concentrations of each would your recommend? Or...

02 October 2021 4,752 3 View

Is anyone able to identify this SF9 cell culture contamination or have you ever faced with something like this on your culture??

I have just found that some of my infected cells got this contamination. I just wonder what those are? It has been 5 days that I have started the infection. Cells are maintained at 28C in ps...

16 September 2021 1,546 3 View

Why do PC12 cells die after 100ng/ml NGF for 72 h incubation?

Hi, I applied on PC12 cells 100 ng/ml NGF and incubated for 72 hours to differentiation. After 72 h, approximately most cells differentiate and then I applied an anticancer drug but all of the...

14 September 2021 4,147 6 View

Protocol Luxol fast blue staining in fixed frozen rat brain tissue?

I have done paraffin-embedded rat brain sections before however in order for all my tissue to be processed the same and for all my antibodies to work, I cannot use paraffin-embedded brain tissue....

13 September 2021 4,268 4 View

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