Nanoparticles toxicology and toxicological assessment?

More Felipe Silva Semaan's questions See All

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View

Can comsol probes be used for stationary studies?

I'm trying to plot while solving comsol probes vs iterations, but the only option i see for x axis is row index from the probes table. And all i can get is a graph with just a point with the value...

22 October 2020 4,618 1 View

RTD Step analysis with matlab?

Hello to all my dear colleagues. I need to get my E(T) curve for my reactor. I already have the measures but I ignore how to procede with the data analysis. I have got Matlab but i am not sure if...

21 June 2020 2,675 5 View

Help about chemical sludge characterization?

Hello. I did run some coagulation / flocculation experiments[FS1] and I have been requested to characterize the sludge. I am ignorant on how to so. I was only requested to do SEM, XRD and Zata...

16 June 2020 8,715 7 View

Split-Hopkinson pressure bar - minimum strain rate?

Does anyone know wich is the minimum strain rate that is possible to get in a Split-Hopkinson test?

20 February 2020 1,835 2 View

3D unstructured meshing in FEFLOW?

Hello, I'm using the latest version of FEFLOW (7.3) and I need to generate a fully 3D unstructured mesh. I already imported a .stl solid shell, wich has no problems at all (i.e. it's a very clean...

31 January 2020 1,365 2 View

In solar heterogeneous photocatalysis, is solar geometry relevant?

My project is supposed to considered solar irradiation as an important variable for heterogeneous photocatalysis. I haven't found any study that considers solar geometry as a variable of study in...

30 January 2020 744 3 View

Massed or interspersed trial in DTT (aba program)?

I would like to hear from fellow behavior analysts on massed trials and interspersed trials. Maybe I did not understand the concept well, so I will post here what I understand by each type of...

26 November 2019 8,433 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

Brian Lee

Japanese medaka (Oryzias latipes) have been used in bioassays with various nanoparticles https://scholar.google.com/scholar?q=nanoparticle+toxicology+medaka&hl=en&as_sdt=0&as_vis=1&oi=scholart

Susanna Gevorgyan

Felipe Silva Semaan , there are various in vitro assays and in vivo methods for assessing nanoparticle toxicity. Assay/method choice depends on the model (cell culture, animal model) and the level (cell, protein structure, DNA, etc.) at which nanotoxicity will be evaluated.

Here are a few examples:

- Cell viability - MTT assay, WST assay

- Cell membrane integrity - LDH assay

- DNA damage - Comet assay

- Protein structure - CD, Cryo-EM

- ROS level measurement - DCFH assay

Find more assays/methods in the publications below:

Chapter Standard biological assays to estimate nanoparticle toxicity...

Article Nanoparticle Toxicology

Chapter In Vitro Methods for Assessing Nanoparticle Toxicity: Method...

Good luck!