What is the maximum size of amplicon amplified by pfu polymerase?

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Kyle Skalenko

yes it should be ok. Just make sure to use an appropriate extension time for your amplicon.

Mateo Glumac

It should be fine. You need to test elongation time and optimal temperature. For PFU I usually use 1 min elongation for a 1 kb fragment.

John Schloendorn

I think it's borderline. You will be more likely successful with Phusion.