Can i apply bell method to equilize the histogram in ImageJ, please let me know the way if that can be done ?

This question is regarding the use of imageJ, I want to apply the same effect to my image that can be done by the command "ret = IpHstEqualize(EQ_BELL)" in image pro premier. I have imageJ only...

01 November 2020 5,660 1 View

Hello dear all. How will Epoch, Batch Size and Iteration be differenciating to each other and what is the correlation among them?

In respect to a Neural Network.

30 June 2019 9,728 3 View

How is correlation different from covariance ?

I need to implement a feature selection method for my dataset and I am asked to use feature selection based on covariance. Therefore I want to understand whether the feature correlation method...

28 May 2019 2,547 8 View

What is the significance of performance metrics used for evaluating the machine learning models?

Most of the study have used confusion metrics and performance measures like accuracy, recall, precision and f-score for presenting the results and comparing the machine learning methods. What is...

24 February 2019 5,329 3 View

Why we used inverse Fourier transform to get back the image?

why we need to bring back the image from frequency domain to spatial domain. please advice and share some related materials.

05 August 2018 1,934 4 View

Can counterstaining override DAB in Ki67?

To test for proliferative cells, I stained my tissue with Ki67- primary overnight and secondary 30min. This was followed by ABC 30min and DAB (1mL buffer + 1drop chromogen) for 30min. I...

27 July 2018 469 2 View

How to construct CNN Model for given description?

Hello Researchers, can anyone tell me how to generate CNN model for any data (expect image, because hat i have gone through till now, it has been done on the image data only), what should be in...

16 April 2018 4,005 3 View

How does adding buffer in path fixes the set up violation?

13 March 2015 1,438 3 View

Can someone suggest a protocol for HPLC with aqueous plant extract?

I would like to mention that i am using terminelia arjuna aq. extract and want to do HPLC using arjunolic acid as standard.

03 February 2015 3,615 3 View

How can I dock a protein with its cofactor using CLC Drug Discovery workbench so that I can find all the interactions individually in binding sites?

How can I dock a protein with its cofactor using CLC Drug Discovery workbench so that I can find all the interactions individually in binding sites?

26 December 2014 5,204 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View