Cell Culture Techniques

Cells in culture or in vitro are a useful model for studying the activity of cells in the whole organism or in vivo. Ten years ago or so cell... | Contact experts in Cell Culture Techniques to get answers

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Questions related to Cell Culture Techniques

EPI of educational institution of india?

need paper of IIT khargaur and energy IIT mumbai

27 May 2024 4,269 1 View

Why energy flow via an ecosystem but matter cycles & relationship between flow of energy through an ecosystem&cycling of nutrientswithin an ecosystem?

Why does energy flow through an ecosystem but matter cycles and relationship between the flow of energy through an ecosystem and the cycling of nutrients within an ecosystem?

23 April 2024 8,119 0 View

Why does the doxorubicin in pbs solutions are giving variable spectrophotometric readings at similar concentrations and similar pH?

Variable readings at same time point and similar solutions

22 April 2024 707 0 View

What is the most effective protocol for extracting cells from breast cancer tissues? Which enzyme is considered ideal for this purpose?

I am currently conducting my PhD research aimed at isolating and culturing cells from breast cancer tissues for further molecular and cellular analyses. Effective cell extraction from tissue...

12 April 2024 4,344 4 View

How to optimize the PCR reaction for DNA extracted from decomposed tissues?

Does anyone have any tips for optimizing PCR reactions with low-quality DNA samples? My interest is in the identification of some species of bacteria using specific primers for each species. I am...

04 March 2024 926 1 View

Sample size calculation for validating a new test against gold-standard?

Dear Friends! The gold-standard for identifying an allele for human leukocyte antigen is SBT or sequence-based typing. If I devise a new PCR test or LAMP test, how many known positive and negative...

17 February 2024 7,890 1 View

What happens to natural extracts (mainly antioxidants) in very alkaline environment?

Hello everyone, Im concerned about the bioactive properties of different natural powder extracts (botanicals and mainly rich in antioxidants) in a very alkaline environment, specifically during...

30 January 2024 1,480 5 View

Is role of sodium bicarbonate under rated in cardiopulmonary resuscitation ?Does it need to be added in ACLS protocol ?

Resuscitation drugs become ineffective during severe metabolic acidosis ...why can't bicarbonate be a part of ACLS protocol

23 January 2024 5,059 3 View

What would the effect of Potassium Formate be on corrosion?

Hello, I was testing the corrosion rate of Aluminium when dissolved in a highly concentrated potassium formate. Now I know corrosion rate is influenced by the presence of dissolved oxygen as well...

19 January 2024 5,385 2 View

I am growing algae on nutrient agar, it is showing bacterial growth only. Where am I doing wrong? Can't find the cause?

I have been growing spirulina algae on Nutrient agar. It only shows bacterial count, not algal growth. Even I have done plating on algae agar, it is not showing any growth. In initial days, I have...

15 January 2024 8,839 0 View

Why do the synthesized cuprous oxide nanoparticles agglomerate seriously ?

I used the sodium citrate method to add sodium citrate to the copper sulfate solution, and then added sodium hydroxide and ascorbic acid to synthesize cuprous oxide nanoparticles. Cubic...

14 January 2024 9,473 2 View

How do you pretreat olea africana seeds before sowing?

olea africana germination

11 January 2024 6,818 3 View

Is it possible to carry out molecular dynamic simulation and obtain RMSD curve/plot using AutoDock Vina (AutoDockTools)?

Binding sites along with binding energy can be easily determined using ADT. However, MD simulations have been reported by researchers using Schrodinger Desmond. So I was wondering if there is a...

29 December 2023 176 6 View

Is it possible/safe to freeze 1cma in diameter hydrated hydrogel in ethane?

I want to plunge a 1cm in diameter hydrated hydrogel in ethane for fast freezing. I was wondering if anybody have experience with this and whether dealing with ethane is dangerous? Is there any...

12 December 2023 9,228 0 View

How can I make my nanoparticle size smaller?

Hello! I'm a 1st year Masters student working with nanoparticles. I've been trying to formulate nanoparticles for awhile now and I've been struggling to get my particles to its true nano-size...

06 December 2023 8,912 3 View

How do I extract statistics from ResearchGate as against list of 25000 articles having DOI?

Explanation: Let I have downloaded 25,000 from Scopus database. I have all DOI in a csv file. Now what is the procedure to get RG statistics (read, citation, recommendation etc) in excel file. I...

03 December 2023 9,664 1 View

Which microorganisms enrich the soil with nutrients and role of microorganisms in maintaining health of organisms and ecosystems?

Which microorganisms enrich the soil with nutrients and role of microorganisms in maintaining health of organisms and ecosystems?

28 November 2023 9,470 3 View

Is it possible to measure extracellular polymeric substances (EPS) in water? If yes, how do I exactly measure EPS in water?

Although there are several papers regarding EPS measurement in sludge, it is quite confusing. Like exactly what need to be measured , and how is that calculated. I would appreciate if someone...

25 November 2023 7,709 4 View

How can I measure protein concentration when my buffer (media) is colored?

I am expressing a extracellular protein and the media is colored. So when I used nanospectrophotometer, for blank (media) it showed high absorbance >340 nm and couldnot measure it. So I diluted...

17 November 2023 1,863 0 View

What are the characteristics of paleocystodinium dinoflagellate species?

What are the characteristics of paleocystodinium dinoflagellate species? cyst type,archeopayel, epiteca, hypoteca,periphragm,endophragm,...

11 November 2023 9,185 2 View

How does DNA strand denaturation occur in LAMP?

I don't understand how BST manages to denature the DNA strand or how the primers bind to a double-stranded DNA in LAMP reactions. A constant temperature is applied, but for BST to displace the...

08 November 2023 7,995 2 View

Can a conference paper be submitted to a journal?

If some conference papers are weak, is the journal's impact factor affected?

07 November 2023 4,220 3 View

What is the culture conditions of scc-9, fadu and detroit cell lines ?

I have tried different medium complements for each cell lines but there is a contamination that i don't know. So i couldn't expand them in culture. Which supplement i should use for each cell...

06 November 2023 3,379 3 View

Why Oleic acid model treated 24 hour FBS free DMEM for induce NAFLD ?

Most of paper refer this model to induce NAFLD however, why most of method followed DMEM without FBS to initiate NAFLD

02 November 2023 3,470 0 View

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