I have to do Annexinv/PI flowcytometry but the prolem is that we have no flowcytometer in our university and I have to take my samples to another university in another city. I have to fix my cells and keep them in -20 centigrade. I would be thankful if anyone could give me the exact protocol for harvesting and fixing my cells to have good results.
I assume you'll end up fixing your cells in PFA or buffered formalin, which typically does a good job. You should be fine keeping your cells overnight at 4degC if needed and then transporting them on ice the next day (or transporting on the same day).
In my laboratory, we usually use trypsin-EDTA from SIGMA (59418C Sigma).
Best.
I think, you can use Trypsin 0.025 with EDTA for harvesting cell. Fix with buffer formaldehide 4 %.
https://www.researchgate.net/profile/Al_Munawir
good luck.
You could try looking through this journal---has detailed stepwise protocols: http://www.currentprotocols.com/WileyCDA/CurPro3Title/isbn-0471142956.html
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