I have use Trizol method to extract my RNA from cancer cells. The A260/280 ratio range is within 1.8-1.9.
Would that be possible to increase the value from 1.8 to more than 2 by re-precipitating the RNA in DEPC water with isopropanol then wash with 70% ethanol?
see the links useful information to improve your RNA Purity
Short answer, yes. But you'll lose some of your RNA (around 30% in my experience).
However, unless your downstream application is incredibly sensitive, I wouldn't worry about your 260/280 at all: 1.8 should be fine. What are you planning to use it for?
I generally worry much more about 260/230 ratio, as this is more likely to affect downstream reactions (though again, even for this, 1.8 should be fine).
I also think that 1.8 should be fine. If you are using a Nanodrop, be mindful that the shape of the curve Abs=f(wavelength) is also very important. Contaminants will affect your ratio. More details in the link below:
http://www.nanodrop.com/Library/T009-NanoDrop%201000-&-NanoDrop%208000-Nucleic-Acid-Purity-Ratios.pdf
Not in my experience, no.
260/230 (rather than 260/280) is more important for RT PCR, since low 260/230 ratios usually indicates guanidium contamination (chaotropic salts are not good for reverse transcription reactions).
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