Does anyone have any good or detailed protocols on developing radiation-resistant cancer cell lines? We are planning to generate such cell lines to study the mechanisms behind radiation induced resistance. Any advice welcomes.
I would like to add that multiple signing pathways contribute to "radiation resistance" in human cancer cells. Therefore, one approach may not be sufficient to "generate" resistant cell line. The best approach will be to collect patient tumor samples, who received radiation therapy and establish primary cell cultures to screen to identify pools of cells in the primary cell culture that respond to a particular pathway identifier.
First of all, I think that no one has a proven method to generate radiation resistant tumor cells.
However, A simple method could be examined, which is:
Culturing tumor cells with media enriched with growth factors, proinflammatory cytokines and excess serum, then exposed the cells to gradual increase of radiation doses, for sure the viable cells would be resistant to radiation.
(like adaptation to chemotherapy).
Cells before and after radiation courses have to be studied on genome level to detect gene mutations responsible for radiation resistance.
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