Please suggest appropriate cancer cell line for Farnesyl Transferase Inhibitors
What will be the appropriate antibody to evaluate cMYC expression in HELA cell line?
11 December 2018 8,382 5 View
Please suggest most appropriate PDB ID for MD simulations for cMYC G-Quadruplex stabilizing ligands?
11 December 2018 4,701 4 View
MD-Simulation tools for Windows: Please suggest me free and efficient tool for simulation ligands with proteins and Nucleic acids sequences in Windows Interface?
11 December 2018 8,311 3 View
Please suggest MD simulation software for windows interface.
10 November 2018 7,265 1 View
Dear Fellow Researchers, I would like to know which cancer cell line will be more appropriate for screening of G-Quadruplex stabilizing agents
07 August 2018 2,798 4 View
Dear All, Please share your experiences on thiazole displacement assay method to evaluate G-Quadruplex stabilzing agents?
02 March 2018 4,073 2 View
I did installation of guass view 03 in my windows based system but it is showing error of faile to establish temp directory. What may be the error please resolve my problem
31 December 2014 4,546 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View