Hi everyone. I know that the L929 has been originated from a normal cells, however, I am a bit suspicious about L929 cells available in ATCC whether it is normal or cancer cell line? I would appreciate any help in this regard.
This cisplatin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (ECACC catalogue no. 85011425) to increasing concentrations of cisplatin in vitro. L929/R cells can be used in the development of novel anti-cancer treatments. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.
Based on your question and description on the L929 clonal cell line from the ATCC, it is likely to be an immortalized cell line (most likely spontaneously immortalized). The cell line if maintained for a long time properly, cells may not be transformed and tumorigenic.
Not only is this a non-cancerous cell line, but it is also the oldest continuous cell line having beeing established in 1948 by Earle.
See:
https://web.expasy.org/cellosaurus/CVCL_0462
Best
Amos
responses above likely correct, but depends on how your particular cells have been handled. if you have just got them from ATCC they are probably not transformed. if they have been in your lab for a long time, it is possible they have spontaneously transformed. you can do a growth in soft agar assay with a positive control tumor cell line.
I agree with comments from Amos and Harold. I did not make it clear that if cells are not maintained properly (including at at right cell density and in the presence of growth factors), these cells tend to undergo transformation spontaneously. Therefore, the assay Harold noted would be desirable.
Thanks a lot for your valuable and helpful comments.
Best Regards
Ali Akbar
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