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Hi everyone, For mAb purification process we performed UF/DF as a final step. Firstly we used small scale cassette (88 cm2 ) as a trial and we did not observe any recovery decrease. Then we pass...
09 January 2023 3,251 12 View
We are trying to isolate primary human monocytes from peripheral blood. Following PBMC isolation with Ficoll, we want to enrich monocytes by herence. We culture PBMC with serum-free 1640 for 2-24...
05 January 2023 2,858 3 View
I am searching for the best approach to statistically evaluate differences in cell cycle distribution between different cell populations. We are analysing the cell cycle distribution, by staining...
04 January 2023 7,650 5 View
2022 was a great year for most of the researchers according to my experiences, and there are many skills that we have learned! According to your experiences, what is the best one?
03 January 2023 5,373 2 View
I want only Calcium metal thin film by evaporation. The color of metal granules is some blackish spots with metallic color. It is stored in an inert atmosphere.
01 January 2023 3,982 1 View
We used polyplus Jetprime to transfect GES-1 cells (one of the gastric epithelial cells). We have evaluated transfection efficiency by western-blot, the target gene have overexpressed...
29 December 2022 6,492 3 View
First of all, I have narrow knowledge about antidiabetic assay as this is my first enzymatic test and I have no one to refer. I have a method but when I tried to google every each of the method...
28 December 2022 2,293 2 View
I do run for 2 sample real time pcr .. their melting curve for one sample show not available tm what this mean ??? Its ct value is 31 thanks alot
28 December 2022 4,620 1 View
Hello, I am struggling to find literature that describes using tamoxifen long-term and its potential side-effects. We are using a model that requires allele deletion (flox/+) via tamoxifen of...
28 December 2022 7,459 1 View
Can anyone please suggest a detailed protocol for tumor sample collection from breast cancer patients? What necessary precautions should be taken?
24 December 2022 6,783 3 View
In a hypothetical single-cell eukaryote, SRP and translocon are both encoded by a single gene. If someone plans to produce a secretory enzyme more efficiently by over-expressing SRP and translocon...
23 December 2022 7,557 3 View
I am a student studying modeling memory devices. I have a question about stepsize for transient simulation in Sdevice. For example, transient simulation is performed from 0 to 1 second. 0.0 to...
21 December 2022 5,098 2 View
With the contents results I can indicate if the CYP 450 is induced or inhibited, or do I need another essay to indicate activity?
20 December 2022 9,937 4 View
I am working with the human LHCN-M2 myoblast cell line which I culture on 0.1% gelatin coated plates. For the myoblast stage this cell line works fine for attachment and growth. However, when I...
19 December 2022 1,593 3 View
This cysteine-peptide is used as vaccine and is being subcutaneously injected to mouse. As DTT is toxic to mouse, what conc. is acceptable?
16 December 2022 9,129 1 View
hello, I've been developing a method on a c8 fortis column. the 4 peaks had excellent peak shape (tail=1 sym=0.9) then when it had an injection from an empty vial multiple times it started to have...
16 December 2022 2,609 5 View
Hello guys, I was wondering what is the right way of laying down a lid is when working inside a laminar flow hood, BSC or isolator. Normally, if a cap of a container needs to be laid down, I...
13 December 2022 4,281 3 View
I am used to pre-incubate the test compounds with enzyme for durations ranging from 15 min to 45 min before adding the substrate and initiating the reactions. However, in process of designing a...
13 December 2022 3,637 2 View
Hello everyone! Can anyone provide a TCAD (Sentaurus) p-gan HEMT model that takes into account the voltage drop across the depletion layer METAL/pGaN?
12 December 2022 4,750 0 View
Initial fluorescence emission intensity is quenched with the addition of an analyte, with time, gradually fluorescence intensity increased. What may be the reason?
09 December 2022 4,755 1 View
Hello everyone, I am trying to do simulation for (Id vs Vd) off state breakdown voltage of AlGAN\GaN HEMT in SILVACO TCAD. I am not getting correct result . I have also attached the code. Please...
09 December 2022 5,767 2 View
Hello everyone, We're working on the cardiomyocyte nuclei isolation from the snap-frozen heart, and we are still facing some problems when it comes to determining if they're good/bad nuclei under...
08 December 2022 7,626 2 View
I'm trying to understand the best way to calculate the spectral slope from absorbance data on dissolved organic matter (DOM). The following formula can be seen in a lot of articles (e.g. Helms...
07 December 2022 276 0 View
I want to isolate mesenchymal stem cells from human bone marrow Do you have troubleshooting and GMP condition for mesenchymal stem cell isolation from human bone marrow?
07 December 2022 8,423 1 View