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Questions related to Buffer
Hi, I will be probing a phospho antibody on my niterocellulose membran. I do blocking by 5% BSA. But this time I accidentally added 5% milk fat and blocked for an hour. How can I remove my...
10 March 2023 7,853 2 View
Hi! I'm using an R program and I have a large table where I have UTR sequences. I would like to parse these sequences from within R (or python) for secondary structure prediction and finding...
09 March 2023 1,833 2 View
I am doing hplc analysis and recently purchased a new column because the old one has degraded. Can one simply install the new column and start straight away with running standards or is there a...
09 March 2023 3,440 4 View
What is the reason for the activation of the antioxidant system but the downregulation of lipid peroxidation products such as malondialdehyde (MDA), and is it a disorder of lipid metabolism?
06 March 2023 7,900 5 View
I have constructed a gene knockout in Candida albicans via the introduction of a premature stop codon in the ORF. I have repeatedly shown that the respective protein is no longer being expressed...
02 March 2023 1,335 1 View
I want to know more about the products EasySep™ Human T Cell Isolation Kit and EasySep™ Human B Cell Isolation Kit. Requirement is to use it for CDC crossmatch using eosin staining and Formalin...
02 March 2023 9,321 2 View
Hello! Does anyone know the enthalpy of reaction for the dissociation of lactic acid to lactate ion and hydronium ion? Is this simply the heat of formation?
02 March 2023 5,629 0 View
Hello. I have a problem with our in-house designed rt PCR for Cutibacterium acnes. Primers and probes were designed as part of mPCR. While testing each set of primers (monoplex) for LOD we are...
02 March 2023 4,354 1 View
Hi there, After constructing a metastatic cancer model, I would like to look at autophagy markers in C57BL/6 type mice. Which method should I choose other than WB and IHC? Thanks
02 March 2023 2,714 2 View
Will there be anything called the dominant negative effect
01 March 2023 564 0 View
Hello everyone, would you please suggest me the optimum seeding density of SH-SY5Y in 8 well plate to do transfection using lipofectamine 3000? Thanks in advance. Best Ayman
01 March 2023 1,656 3 View
And this absorption rate of TMB by uv vis depends on what factors And whether the oxidizing agent of TMB is also effective on the amount of absorption at 650 wavelength
01 March 2023 9,785 1 View
Hi there, After constructing a metastatic cancer model, I would like to look at autophagy markers in c57bl/6 type mice. Which method should I choose other than WB and IHC? Thanks
01 March 2023 3,717 5 View
I have been working on a research project that involves the extraction of alkaloids and saponins from Balanites aegyptiaca. As you may know, this plant is rich in polysaccharides, which makes the...
28 February 2023 4,732 3 View
I've read that most flow cytometers use the blue 488nm laser to make FSC and SSC measurements, but what about the detector? Is it a specific range of wavelengths like most filters on flow...
28 February 2023 9,658 2 View
Hello! I need to test anti inflammatory activity of a hydrogel by using protein denaturation assay. We use BSA 5% in distilled water (pH 7.4). We prepared 3 samples (PBS 2.5 mL + BSA 450 uL, PBS...
27 February 2023 5,209 7 View
We are designing a clinical trial with fibre supplementation for humans. Is there anyone with expertise in that area to be involved as a collaborator? Someone with a nutrition background and...
24 February 2023 9,533 5 View
Good day, If protein expression in blood monocytes was low (detected by western blot) and protein level in serum (detected by ELISA) was high? what dose it mean? Note: the protein should not...
23 February 2023 8,477 6 View
It is important for us, that the sample data can be link to the laboratory practices (what is going on with each sample), very good traceability of samples, possible linking to results and analsys...
23 February 2023 401 2 View
Could anyone share a protocol for the isolation of Sarcoplasmic reticulum from mice muscle ?
21 February 2023 4,565 3 View
Hi colleagues, I am planning to study the interaction of a DNA-binding protein with a plasmid DNA sequence by FRET assay in vivo. I want to label my plasmid with FITC and transfect it into...
20 February 2023 7,981 4 View
Hello, We're going to perform a Seahorse assay using XFp 8-well system. The cell density will be 20,000 cells/well. We don't have the Cytation system to perform automated cell counts. As well as,...
20 February 2023 8,979 6 View
I have made gold nanoparticles using the reverse turkevich process. After infusing salt (ZnBr2), I have been getting globural structure of nanoparticles (blue in UV Vis plot) instead of 1D...
17 February 2023 7,945 2 View
I did protein extraction and purification with ADH_pET11a in BL21. The picture of SDS-Page have total and purified ADH bands shows under 35kda. I want to know why band is under 35 . Expected size...
17 February 2023 4,156 4 View