Hello Dear all!
I would like to know if it is possible to have a stable cell line with a response element attached to a luciferase signal, also, Could it be possible to have in the same stable cell line a fluorescent protein that indicates the insertion within the genome of the before mentioned response elements/luciferase at the moment of generating it? (Have in mind that the plasmid used to generate the stable cell line confers a hygromycin B resistance to the cell line, after selection with this antibiotic)
if that is the case would it mean that when looking at the cells in a fluorescent microscope with a suitable laser exciting the fluorescent protein all the cells should exhibit fluorescence?
Thanks in advance for your help
In principle, it is possible. Not sure why you'd want to do it though. Both signals are acquired differently (chemi vs fluorescence) and should have little to no overlap. Luciferase is not fluorescent and fluorescent proteins will emit no light in a luc assay (dark assay). So yould have a bicistonic or p2 like system driving both luc and your favorite fluorescent protein or a dual cassette driving both separately.
Yes, this is possible and is a strategy used in research. One of the examples I am familiar with is Plasmodium falciparum sporozoites that express both GFP and luciferase in order to track the parasite in different assays. A good description is available here: Article Optimization of an in vivo model to study immunity to Plasmo...
- Badges
- Science method