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Hi all, I possess several scFv DNA sequences and aim to generate soluble forms of these single-chain antibodies to assess their biochemical characteristics, such as binding affinity and...
19 December 2023 2,095 0 View
I need cited details on activated carbon preparation?
18 December 2023 2,039 3 View
One of the main challenges of long-term culture *1 month* is degrading the collagen or your matrigel by 1. cellular force during expansion and replication and 2. enzymes such as...
18 December 2023 4,088 2 View
We are interested in CD8+T lymphocytes form pancreas cancer patients. Is it possible to find a pancreatic cancer cell line with HLA class I molecules that could be recognised by CD8+T lymphocytes...
16 December 2023 4,189 1 View
Cats and dogs in a slum area are not healthy.
16 December 2023 8,013 0 View
I tried to express a Large fusion protein (about 223 KDa) in bacteria (BL21de3) in the pet28b vector, but it failed as the SDS PAGE shows just very few proteins successfully expressed. I wonder...
15 December 2023 9,496 3 View
For our LMMP protocol, first I placed the tissues in phosphate-buffered saline (PBS; 0.1 M, pH = 7.4) containing niphedipine for 15 min, then the tissues were fixated in 4% PFA in at 4 °C...
14 December 2023 2,938 0 View
How can l learn what Q value of Indian Journal of Pharmaceutical Sciences? Because no information was given
14 December 2023 9,992 2 View
Hey there, I am working on studying the volcano plume height detection using GNSS RO bending angle, for which I am utilizing the atmPrf datasets from the cosmic-2 satellite constellations....
12 December 2023 2,299 0 View
In one my Articles (paper 406), published in IGC 2015, my name was reflected as Rao,N.K. which is otherwise known as Kutumba Rao Nathala in other articles. so unable to upload in my profile....
12 December 2023 1,822 0 View
Please explain why you think students find speaking difficult. Please explain clearly and simply, how you would help them to improve their speaking skills.
12 December 2023 4,280 4 View
Hello, I've been having trouble recently with my western blot experiments on cell conditioned media. I'm using a home-made 10% SDS gel, as well as a home-made migration buffer and loading...
12 December 2023 9,615 1 View
a soil sample taken from and pld abbey, the soil sample where taken from a room that have a blast-furnace in it, while an x-ray fluorescence gave a concentration of 1.5% of fluor, and an analysis...
11 December 2023 9,305 3 View
I am using Agilent 7000 series GC-MS/MS for the analysis of DTC pesticides such as Mancozeb and Zineb but the LOQ cannot be lowered up to 10 mg/Kg as there is matrix interference from the tea...
08 December 2023 4,803 1 View
LiDAR data collection and processing
08 December 2023 3,762 0 View
The mice were induced to have type 2 diabetes by feeding high-fat food and injecting low doses of STZ.
08 December 2023 5,700 1 View
A company produces PP surgical products from granular raw materials by injection. molding technique. They did not find any weight loss after melting. According to the manufacturer policies, they...
07 December 2023 8,380 2 View
Hello, I am using a dataset that has various variables in currency, values, counts, and percentages. To bring them all to one level I understand I need to take the log values. However, since...
07 December 2023 9,626 2 View
How to Reasonably Weight the Uncertainty of Laser Tracker and the Mean Square Error of Level to Obtain Accurate H（Z）-value?
06 December 2023 8,809 2 View
Which approach makes the most sense for creating a pest map of any insect pest?
06 December 2023 535 2 View
Does anyone know of any data for TEV protease LD50 or other safety information for the protein itself? Thank you!
05 December 2023 7,432 2 View
If we use undoped materials and record their PL intensity, the intensity of the sample rises or decreases after doping. We need a low rate of recombination with a higher charge carrier...
05 December 2023 2,382 1 View
For design in silvaco
03 December 2023 9,676 0 View
I encountered a problem during electrophoresis where I found that the bands were not evenly aligned during protein separation (after migration into separating gel). I also used freshly prepared...
02 December 2023 3,526 3 View