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Does anyone know of a fluorescent protein with an emission max greater than 720 nm? Which is the best fluorescent protein with an emission max greater than 700 nm for making a BRET sensor? I have...
18 May 2023 1,142 9 View
I wonder if there is any available software to design multiplex qPCR assays ?
18 May 2023 9,123 2 View
It appears to be generally accepted among oncologists that Mesna must be administered with cyclophosphamide doses exceeding 1000mg/m^2. I am having trouble finding literature that supports this...
17 May 2023 604 0 View
I have run the simulation but the newbox file doesnot show coordinate. Instead of coordinate it shows nan.please help me to overcome this problem
16 May 2023 7,082 2 View
what makes the difference if we choose metal chlorides or acetates instead of metal nitrates for synthesizing MOFs
16 May 2023 1,628 3 View
problem statement for a research proposal The purpose of this study is to investigate the ongoing question on how consumers are affected by the cost of cellular data especially in South Africa...
16 May 2023 6,696 0 View
Hi, I am trying to demonstrate a protein-protein interaction through co-IP. To IP, I use a mouse IgG isotype control and a lab-generated mouse antibody to protein X. When I do WB, I use...
15 May 2023 2,406 4 View
We usually use the pospho Protein: total protein ratio to evaluate the protein phosphorylation. But what does it mean if we take total protein: pospho protein ratio? Because when I take this ratio...
12 May 2023 7,854 3 View
Hi all, I'm trying to extract total RNA from exosomes using RNAeasy Qiagen. I've tried to use 2x109 EVs, as I found on a paper, but it didn't work. When we measure on Nanodrop, the concentration...
10 May 2023 2,340 1 View
What’s relationship between biodiversity & ecological stability & difference between ecosystem & biodiversity & its importance?
10 May 2023 1,313 6 View
Say for example is 50/50 an acceptable value? After what number should we stop aiming for higher values? Below what percentage of live cells should we abandon the experiemtnas erroneous or...
09 May 2023 9,362 1 View
Hello Researchers I would like to perform the 2D simulation of a PVT system with fins using FORTRAN. I therefore ask for help for anyone who has it or who knows a website where I can find it. The...
01 May 2023 349 2 View
Bonjour, je voudrais avoir vos avis : j’ai quantifié l’ADN extrait par le Nanodrop 2000 à l’Absorbance 260/280 le ratio est de 1,95 mais pour le ratio 260/230 est égal à 0,23. certains auteurs...
01 May 2023 3,229 1 View
Hi, I want to quantify the amount of collagen(s) that got secreted outside of cells to ECM through Western Blotting the media, however I'm not sure which housekeeping gene I can use in order to...
28 April 2023 2,012 4 View
I have export the Silvaco log file into Excel, i have also set up follow the current setting, but how do i know what value belong to it , Only Source, Gate,Drain is label , other i have no idea....
28 April 2023 337 1 View
Does anyone know any antibiotic that inhibit enterococcus but allow other probiotic like bifidobacterium?
27 April 2023 2,979 1 View
I have to CAM assay for some samples. I'm planning to conduct the assay in eggs only, but is it better to conduct it in petriplates? While adding drugs (on eggs), whether we should inject it to...
26 April 2023 7,795 0 View
how to perform cys -cys cyclization of peptide (disulfide bond formation) after linear peptide synthesis , what is reagent used ,which not affect linear peptide sequence
25 April 2023 143 3 View
For fluorescent immunohistochemistry I fixed spinal cords in PFA, incubated in 30% sucrose, froze in OCT, and sectioned at 30um. All my sections are now on untreated charged glass slides that...
25 April 2023 4,414 4 View
Could we autoclave a Guaiacol? If we couldn't autoclave, haw do we sterilize it? Even it couldn't pass through a filter paper. So how?
25 April 2023 5,500 0 View
While using a passive bat detector in the mangroves at Kalba, UAE, we recorded numerous calls which we can't identify. The first ones were a series of clicks with frequencies between 70 and 40 kHz...
24 April 2023 9,428 0 View
1. I got red brown colony but not red brown clear zone. Can we say that it is a positive result 2. Tannic positive results can not be a positive on Guaiacol. Why? 3. Can I autoclave guaiacol and...
22 April 2023 2,008 2 View
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21 April 2023 4,911 3 View
Hello, Now I am looking for the best device for scanning cranial nerves (especially vagus nerve). However, I have not much experience for ultrasound imaging. So, need some recommendations from...
21 April 2023 3,169 1 View