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Questions related to Buffer
the buffer, im concerned with is citrate buffer( using citric acid and sodium citrate dihydride) and its complex compound formation is with iron, its application is being used in the electro...
25 January 2023 5,876 2 View
It goes without saying that if someone has a blood clot in their brain, it should be cleared as soon as possible. An experimental new transducer could help, as it uses swirling waves of ultrasound...
24 January 2023 7,944 1 View
Please, what is the best way to totally remove traces of phenolics from dialysed polysaccharides extracts?
24 January 2023 9,864 1 View
The silicon wafer was used to develop a microchannel with a negative photoresist. After stripping away the old channels, I want to reuse the same wafer. Is it possible? If yes, what is the...
24 January 2023 873 1 View
I am trying to detect endogenous protein for my gene of interest. There is no commercial antibody available for mice. I have tried to detect the protein of my interest with the home made antibody...
24 January 2023 5,845 3 View
Dear all, i am using LTP protocol on mice hippocampus and i am facing recently a new issue: the amplitude of fEPSP is decreasing with time while recording (the recording of 1st slice has no...
23 January 2023 6,090 4 View
This is my first time trying Western Blotting and I need help with managing the unknown protein quantification using a plate reader (SpectraMax/Softmax Pro software). I have an African cichlid...
23 January 2023 8,828 3 View
I need some suggestions and suitable ball mill for mixing the elemental powders of 325 mesh size like titanium, tungsten,carbon,aluminium powders etc.. I need the model, name and brand of the ball...
23 January 2023 6,341 4 View
I am looking to model how expression of a target gene from a state of dysfunction (i.e., knockout; CRISPR-Cas9 induced) to overexpression may influence aspects of neuronal functioning/morphology...
22 January 2023 9,462 2 View
I'm going to transiently transfect subcutaneous pre-adipocytes with His-tagged plasmid DNA [pcDNA3.1(+)] containing ADIPOQ gene with a SNP to study the adiponectin expression. Can any expert...
19 January 2023 8,116 3 View
I used Lowry Technic. I prepared standards from BSA for make up standard curve. And then, ı measured my samples proteins. How can ı find protein contents in my samples?
18 January 2023 2,168 2 View
I have a molecule that changes the color of the media to yellow; when I was trying to use this for the LDH cytotoxicity assay, I had a lesser value than the test. As the compound was changing the...
17 January 2023 3,661 7 View
Hi there, we have to generate a tissue-specific knock-out using the cre loxp system. However, the heterozygote cre line is based on C57BL/6 strain whereas the homozygous floxed mice are on BALB/c...
17 January 2023 9,509 1 View
As additional information, I need information about the number of copy virus from the PCR result by identify the number of RNA.
16 January 2023 1,640 3 View
I have been trying to purify Amyloid Beta peptide using ion exchange chromatography. In the paper I am referring to, they have used DEAE Cellulose in their protocol. I have been using Q-Sepharose...
16 January 2023 2,477 0 View
The goal is to try different denaturing methods for bl21 E.coli however, finding detailed paper on this is challenging as most articles do not seem to inclide the deatils of their denaturing...
16 January 2023 8,003 7 View
In my current bioanalytical method development project, I achieved optimal separation between my analyte (A) and internal standard (IS) using HPLC with fluorescence detection. However, the...
16 January 2023 9,575 11 View
Last week on ResearchGate 3 new cites where identified on one of my papers. 1 from December 2022 and 2 from January 2022. How long does it take for Google Scholar to sync with ResearchGate and...
16 January 2023 8,112 5 View
Working with human serum and plasma samples, after separating from formed elements and making aliquots of the sera and plasma, some of them haven't frozen (around 10% of the aliquots, mainly...
16 January 2023 9,033 2 View
I am trying to develop a HPLC method for the quantification of carotenoids using a C30 column that was bought recently but I am running into some issues. I do not see a peak for the analyte...
13 January 2023 8,942 5 View
Skeletal muscles are stained with the blue color but other half of the picture was stained with red color. Can someone tell me what kind of tissue or cell is it?
13 January 2023 2,919 3 View
Hello, I am setting up experiments with inducible ERT2-Cre mice, where our control mice received vehicle only (peanut oil). Do we need to put the controls and TAM-treated (0.1 or 1 mg per day,...
12 January 2023 5,031 2 View
i have precipitated enzyme by acetone.. when i use coomassie staining in native gel electrophoresis, it couldnot migrate to stacking and then resolving gel. is there is a problem in acetone...
11 January 2023 2,320 5 View
Hi all, I am planning to have the in vitro transcript of my gene of interest which is under the control of CMV-IE promoter. Is there any commercial kits or any established method for that?
11 January 2023 9,285 1 View