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Questions related to Buffer
Hello, I have HisG tag monoclonal primary antibody in my lab that are purposed for western plotting analysis of N-terminal tags with 6x His-Gly tags. Can this antibody be used with C-terminal...
24 October 2023 5,057 3 View
I will be using flow cytometry for my PhD thesis, and it would be very useful to have some recommendations on resources such as books, review articles, or online courses where I can learn how to...
23 October 2023 5,570 2 View
physiology and structure of mitochondria
22 October 2023 2,560 2 View
I am a high school student huhu :( asking for help for our investigatory project, supposedly our parameters to test our textile fiber from grass were: tensile strength, flexibility/stiffness,...
22 October 2023 3,575 1 View
Hi! I am trying to extract protein from brain samples and I want to use 2% SDS lysis buffer, I know the ingredients but I can't figure out the exact amount of those ingredients. Can someone help...
19 October 2023 7,533 3 View
I completed a western blot and during detection, there were black dots all over the membrane on the output. What could have caused this?
19 October 2023 7,677 3 View
I prepared electrocompetents of the AGL1 strain following the "water" protocol: the bacterial culture was resuspended in water, subjected to two washes in water, one in 10% glycerol, and finally...
18 October 2023 4,860 3 View
Hello, we have been struggling with the lots of background in our wb membranes probed with an anti-Streptavidin-HRP from Thermofisher (Pierce 21134). Samples contained biotinilated proteins. Every...
17 October 2023 3,846 2 View
How can I plot a professional image like the image is the attached
15 October 2023 8,061 3 View
do T or B lymphocyte s present in histological structure of ovary ?what is the defence mechanism in ovary ?
15 October 2023 1,604 3 View
Hello, I'm currently navigating the intricacies of preparing A549 cell pellets for Atomic Absorption Spectroscopy (AAS) analysis. Here's a brief overview of my process: Treatment: A549 cells...
14 October 2023 8,077 2 View
Hi, I am currently trying to isolate RNA from whole blood. I have frozen blood samples - preserved in RNAlater stored at - 80. Samples were collected in RNA later (300 ul blood sample and 1.3 ml...
13 October 2023 435 3 View
As Empatica E4 EDA/GSR wristband is discontinued, we are looking for a new device that can be used for scientific research. Ideally, a wristband and no electrodes since we would like to use it in...
10 October 2023 9,389 1 View
I did protein purification from polyacrylamide gel because i want to cut only target size. But, after running SDS for checking purification, there are several bands were shown. Moreover, there...
10 October 2023 4,847 4 View
I need help with a PCR. I have the following primers mecA-F 5′AAAATCGATGGTAAAGGTTGG-3′ / mecA-R 5′-AGTTCTGGCACTACCGGATTTTGC-3′. I made an initial PCR with 12.5 of GoTaq Green Master Mix, 2x, 0.8...
06 October 2023 7,293 2 View
Hi Everyone, I'm currently attempting to use Golgi-Cox staining to visualize dendritic arborization, but I'm struggling to get the full neuronal structure seen in classical Golgi-Cox staining. Has...
06 October 2023 4,343 0 View
Why MOE uses so little "power" capacity of my CPU (Intel Core i7-7700HQ)? I ran a calculation this way: I opened GUI of MOE --> Compute --> Fragments --> Add Group to Ligand. I selected...
04 October 2023 799 0 View
I designed a plasmid to express one specific gene and I inserted the T7 terminator after stop codon of gene sequence… and when I checked it was reversed. I am wondering if it will work or not, and...
03 October 2023 3,224 2 View
How could politicians and scientists better work together to address issues in our world? For example, can Researchgate provide opportunities for politicians to get involved in some sort of...
03 October 2023 9,390 6 View
I am using a UPLC-ESI-MS (single quad) to quantify a corticosteroid in negative mode. The molecular weight of the compound of interest is 392 but the m/z after scanning with the largest peak is...
02 October 2023 9,495 2 View
Hello Everyone, I am trying to extract viral RNA from infected Thp1 cell cultures. I thought I could use TriZole for cell homogenization. can I combine two methods: trizole to keep the samples...
02 October 2023 3,144 3 View
Cerebroside and ceramide. Esi-ms in positive mode.
02 October 2023 2,088 1 View
I have got some files from GC-MS analysis with .raw extension, but I have got the masshunter software that cannot open these files, but only .D files. Do you know how to convert the files in...
02 October 2023 8,530 1 View
I am designing primers for my study. I couldn't find answer to my question anywhere. I will try to see the effects of a drug on transcript levels of different genes. I intent to cover all mRNA...
01 October 2023 1,489 3 View