What are the ways to confirm the edited gene through CRISPR system?

More Sedighe Setayesh's questions See All

What selectable marker genes do you suggest instead of hygromycin in the gene knockout project in rice?

14 May 2024 2,680 0 View

Abnormal changes in texture and color of chicken meat?

Dear Sir and Ma'am, We faced a problem in the industry that couldn't be solved despite running several tests. "When using chicken thighs for making sausages, we noticed weak tissue in certain...

11 January 2024 4,415 0 View

After designing gRNA, how should we make it?

After designing gRNA, how should we make it? I need an article, site or instruction on this please

26 September 2023 9,258 0 View

What are the ways to confirm the edited gene through CRISPR system?

Please suggest me some articles or sites about this topic.

22 September 2023 2,553 1 View

What is knowledge gap in tourism research?

what is the knowledge gap in tourism research?

10 May 2023 4,024 4 View

How can I find proteins in Artemisia annua plant that are involved in primary or secondary metabolites?

I have to draw a phylogeny tree using their complete sequence on the NCBI website

19 April 2023 8,745 0 View

How to get consistent result from CD spectroscopy?

I am doing CD studies on amino acids by measuring the CD intensity (quantative) for various concentrations of amino acids both when they are pure and when they have been used as precursors for...

07 December 2021 9,234 7 View

How to differentiate the emission peak of a compound from the emission peak of the light source(instrument)?

I am having trouble recognizing whether the emission picks that I am seeing are from the light source of the instrument or from my sample?? is there a way to better discriminate them? (I am not...

21 November 2021 9,368 3 View

Hi, I am looking for this book "introduction to magnetic material" by Cullity?

Hi, I am looking for this book "introduction to magnetic material" by Cullity , how can I find it?

16 January 2020 229 2 View

Hi, I am looking for a solution manual of this book "introduction to magnetic material" by Cullity , how can I find it?

16 January 2020 3,695 2 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Why knockouts for same genes from two different gRNA are showing different result?

Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.

25 July 2024 8,313 1 View

Is possible to edit a gene present in a plasmid by Crispr Cas9?

Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...

22 July 2024 7,073 2 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

What is the most effective method for knocking out a gene in Candida albicans?

I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...

15 July 2024 3,977 1 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Are there any information about protocols of CRISPR in leishmania strains?

I am looking for a CRISPR protocol for application in leishmania strains

08 July 2024 9,732 1 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

There is no colonies after Ligation reaction and transformation ?

I am currently investigating the CRISPR 9 case to target my gene of interest for knockout. The vector being used is lentiCRISPR v2puro. I digested and dephosphorylated 5ug of the lentiviral...

03 June 2024 2,877 7 View

How can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are there any ways to increase the rate of HDR?

Now, I am using CRISPR/Cas 9 system to make a point mutation at THBD gene. I am wondering how can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are...

30 May 2024 9,993 0 View

Marcos Antônio de Oliveira

Hi!

There are different ways to do this. The most common is the T7 endonuclease assay.

I also use the specific tool to determine if you have INDELS in your sample (https://tide.nki.nl/). This software is amazing.

Sedighe Setayesh

Hello. Thank you very much for your help

For a class seminar, I need to explain the methods of verifying the edited gene. I wanted to know how many ways there are to confirm this

Enrico Virgilio

Sanger sequencing comparing the original sequence and the putative edit would be ideal