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Questions related to Buffer
I want to do a drug screening assay in a 96well plate and test protein level of cytokines with elisa.
03 August 2023 2,520 2 View
i isolated the phage and put in SM buffer and than in 3ml brucela broth add 100ul bacteria and 100ul phage from SM buffer incubated for 48 hr at 42 hr than centrifuge at 10000rpm for 10 min than...
02 August 2023 2,131 3 View
I am interested in looking at published single-cell data to verify my gene of interests. Any advice would we much appreciated.
01 August 2023 9,962 1 View
I've tried to simulate breakdown voltage of a AlN/GaN HEMT. But it always fail to complete when calculating Impact Gen. This is my Impact model: impact SELB region=4 AN1=2.11e9 BN1=3.689e8...
31 July 2023 3,228 0 View
I tried to make HEPES buffer solution by using C1V1 = C2V2 formula. But the prepared buffer isn't okay. could anyone please suggest me how to prepare 50mM 10mL HEPES buffer from 1M HEPES buffer...
31 July 2023 6,933 10 View
I want to deplete CD3 T cell from PBMC, how can I do that without magnetic bead separation?
31 July 2023 1,172 5 View
I have precipitated out the protein using acetone from the alkaline-base buffer (NaOH + SDS+ EDTA + Beta Mercaptoethanol). Desiccated Precipitates weigh more than the sample i.e for 2 grams of...
28 July 2023 8,729 4 View
Hi, community! I have been using 12% SDS gel to separate my protein of 57kda since last year and my SDS was working just fine. Lately I have been struggling as my protein is not entering the...
26 July 2023 4,916 2 View
Hi everyone, I've tried to transfer initially using a 20% methanol transfer buffer using 250mA max current for 70 mins, all I got was transfer of mid-high range proteins, I suppose all proteins...
26 July 2023 1,832 4 View
Can someone please share a copy of the article by C. H. G. Arden-Clarke - Population density, home range size and spatial organization of the Cape clawless otter, Aonyx capensis, in a marine...
26 July 2023 5,643 4 View
I am working on an algorithm for dominant speaker selection in a closed proximity cluster of smartphones (iOS and Android) with arbitrary positions. The smartphones act as clients, and one of them...
25 July 2023 1,373 0 View
I measured the absorbance of CBD (1mg/ml in methanol) diluted in HEPES buffer (0.2M NaCl and 20mM Hepes pH 7.5). I have attached a picture of my results - however it does not resemble what is...
22 July 2023 7,015 2 View
I have to perform a new experiment. I have to stimulate MDA-MB-231 and THP-1 differentiated with a recombinant protein. I red that cells have to reach an 80% cell confluency before stimulation...
20 July 2023 6,851 4 View
Hi all, I got a .sds file from a colleague. We'd like to look back the set up of the qPCR he ran back then, but I don't have the right software to open it. I looked up on applied biosystem...
20 July 2023 7,405 0 View
Hey dears, I'm getting ready to create a genetic construction where I'll tag my protein with three flags. I'm aware that I must place it in either the C or N terminal, but I'm not sure which...
18 July 2023 492 2 View
What would be the labels for x-axis and y-axis. I had mesenchymal stem cells from 6 different donors treated with salmonella (high conc, low conc. and control)
17 July 2023 8,774 3 View
hello. In our lab, we incubate e.coli for 16 hours at 37 degrees Celsius and 130 rpm. We then streak and incubate at 37 degrees Celsius and 225 rpm and process IPTG when the O.D. reaches 0.6. I...
16 July 2023 609 0 View
I have two synthetic peptides (20-25 AA length) with terminal cysteines that I have been trying to conjugate to maleimide dyes. The maleimide-conjugation protocol has seemed easy enough, but I'm...
14 July 2023 1,429 4 View
Hi everyone, I have a question regarding indirect FACS protocol. I'm using an unconjugated antibody which is biotin-tagged and PE-streptavidin as a secondary antibody. What should be their...
14 July 2023 4,582 3 View
Why natural polyphenols (like proanthocyanidins from berries) decrease their antioxidant activity in a lipid environment? vs an aqueous environment? I know that existing research suggests that...
13 July 2023 4,457 2 View
Why is the ecosystem unstable and role of biodiversity in ecosystem function and stability biology?
13 July 2023 1,161 0 View
What is stability of ecosystem function and which level of biodiversity has an important role in maintaining the balance of the ecosystem?
13 July 2023 367 0 View
Entellan for floursence microscopy as mounting medium?
12 July 2023 810 2 View
I am doing absolute quantification RT-qPCR for the beta actin gene. I start with RNA, turn this into cDNA and use that for qPCR. I am going to get a custom oligo of the PCR amplification product...
12 July 2023 518 4 View