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While isolating phages from natural sample, I got different plaques indicating mixed information. I want to isolate a single phage so started culturing with only 1 plaque. I took out single plaque...
28 March 2023 8,939 6 View
hi, I am currently isolating DNA from tissue. I am trying to analyse the DNA integrity by running a gel electrophoresis experiment. I'm currently using 1xTAE buffer, 0.5% agarose gel, 60 voltage...
27 March 2023 3,753 3 View
When humans ingest sodium alginate, do they absorb the sodium (and would it need to be calculated into their total sodium intake for the day)? Is it safe for those ingesting it as a prebiotic but...
27 March 2023 6,302 0 View
I have conduct alpha glucosidase inhibitory assay. But i'm confuse why the absorbance of the sample was highest than blank (without sample), as well as using acarbose as positive control. Anyone...
25 March 2023 1,145 0 View
Hello everyone, for my research project, I am doing synthesis with polymer (PEG) and I confirm the formation of the product via NMR. But for me, I don't really trust NMR and it cannot confirm me...
24 March 2023 7,620 3 View
I found many papers that claimed that AB1-42 1uM is not enough to significantly increase Caspase 3 activity and significantly reduce Bcl-2 expression in SH-SY5Y cells but I also found some papers...
24 March 2023 3,733 1 View
My study is on lipase and I planned to used p-NP palmitate method as the activity assay. The recipe includes Tris HCl buffer pH 9 + Triton X-100 + isopropanol. I stirred the mixture with heat then...
22 March 2023 3,268 4 View
We were culturing murine B16 melanoma cells and were trying to prepare a cell suspension after detachment of cells using trypsin. However, cell clumps were formed. Did we make something wrong? I...
21 March 2023 6,002 4 View
Iam unable to get any bands.. So it will be helpful if i know about alternatives..
21 March 2023 5,000 10 View
I cultured normal Hela cells. I subcultured them one day, then found some dots in the media the next day. Then I saw the dots are more and more, among the media and in the body of the cell, and...
21 March 2023 2,195 3 View
To find the oligomeric nature of the purified protein, I performed BN-PAGE. I am getting 9-mer and 6-mer and lower bands but they are degraded. alternatively, we have also done SEC and the results...
21 March 2023 8,272 0 View
Hi! How can I add technetium atom in AD4_parameters.dat file for the ligand containing technetium in the molecular docking i will perform with Autodock
19 March 2023 9,368 1 View
I have unstained FFPE tumor tissue, with the area of interest graphing on the back of the slides. The goal is to scrap off the area of interest, then extract both DNA and RNA. What's the good...
17 March 2023 1,956 1 View
Hi Everybody, can you help me? In February 2023 I happily got two peer-reviewed papers published (see below), which I previously had archived in December 2022 on preprints.org. Meanwhile the...
16 March 2023 6,523 1 View
I am trying to perform a silylation reaction and need to find a way to quench the excess TMSCl. I am particularly concerned with the evolution of white vapor after the completion of my reaction.
16 March 2023 556 0 View
I collected forest-tropical soil samples and immediately put to in LifeGuard Soil Preservation Buffer. I followed all the instructions given by the company. After two weeks, we returned to the lab...
15 March 2023 1,941 0 View
I want to show protein-DNA conjugates in a gel. The proteins were first covalently linked to a single-stranded DNA-oligonucleotide. Then I annealed a strand to the first oligonucleotide and...
14 March 2023 5,577 3 View
Solution is not converging after gate voltage reaches 1.34 V and Id is 200mA
14 March 2023 2,742 1 View
we have synthesized anticancer drugs which are toxic to cancer cells. but it is desirable that such drugs are non toxic to normal cells. pls provide me a list of normal cell lines where such...
14 March 2023 2,475 4 View
F4/80 is used as a marker to identify murine tissue/peritoneal macrophages. In our working groop we isolate monocytes from rat tibia and femur and differentiate them subsequently into bone...
14 March 2023 8,336 3 View
I want to perform a MD simulation in LAMMPS for a water droplet impacting a rough Copper surface at an assigned velocity and then nucleate this droplet into ice. I have made the droplet...
14 March 2023 8,537 3 View
While determining Pb, Ni, and Cd in a soil sample using AAS, I obtained some results that were lower than the blank result. The sample was digested using 2M HNO3 and diluted to 50 mL. 1. Is it...
10 March 2023 3,675 10 View
There was a problem with using the kit FFAR1 Reporter Assay Kit (Cayman Chemical). The kit is based on transfection of cells HEK293T with a DNA complex of FFAR1, altered G-protein, and secreted...
10 March 2023 8,019 0 View
I have to do profiling of amino acids through HILIC chromatography, so I have Zorbax NH2 Column. So, Can we use the Zorbax NH2 column as the HILIC column for the separation of amino acids?
10 March 2023 689 4 View