Are Air-cleaners effective in controlling COVID-19 in indoor environments ?

The use of air cleaners is recommended by some experts to filter the air in indoor environments like home, office, school and other publich places. They have to be equipped with HEPA filters and...

12 November 2020 4,817 3 View

COVID-19 and Geography. What are the levels of impact and who will survive?

Latitude, climate, humidity, infrastructure, health care systems, socioeconomic situation, health condition of the patient and pre-exposure to vaccinations like BCG, antimalarial drugs. Are they...

21 April 2020 6,869 8 View

How to model robot dialogue model according to emotions?

I'm designing a user study on robot-human communication and I want to design the robot speech according to human feelings. I did a research to understand how human feels from speech, and I want...

05 January 2020 7,107 3 View

Is it OK to do a user study with people in a language other than their mother language?

I'm planning to do a user study in Korea, and the study itself is in English. I wanted to hire people with certain English skills, but I was wondering if this is acceptable in academic standards?

05 January 2020 9,293 5 View

What are the main criteria to evaluate an exploration project's success?

How should an exploration project be evaluated as successful or unsuccessful? What are the main criteria? Exploration of an unknown deposit based on recent protocols or standards (such as JORC or...

28 December 2019 460 19 View

What polymers do PNIPAM or Poly(N-vinylcaprolactam) is Miscibile with?

Can you share any relevant atricles? Can you share the curremnt knowledge in this? Any application examples other than gels / membranes? Thank you

11 September 2019 3,926 1 View

How do I continue with my analysis if the Box's M and Levene's tests are significant in MANOVA?

I am trying to run a MANOVA, but the Box's M and Levene's tests are significant. Does this mean I can not run a MANOVA? Is it OK to continue if I choose to report Pillai's trace in stead of Wilks'...

01 January 2017 4,851 11 View

How to make Tris Succinate buffer (0.05M, pH8.2)?

How to make Tris Succinate buffer (0.05M, pH8.2)?

25 October 2016 5,036 3 View

Any suggestions about my struggle with fabrication of silver coated core shell nanoparticle?

29 August 2015 5,565 8 View

Is Matrigel or GFR-Matrigel better for transwell invasion assay?

I will be working with NCI-H460 cells.

02 February 2015 9,969 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View