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We want to determine curcumin in mouse plasma after subcutaneous administration of some formulations. The idea was to perform the determination by HPLC-MS. However, we have been having a problem...
11 July 2023 4,069 2 View
I recently performed the glycidylation of some polyphenols. And I just read a paper (Doi: Study of the O-glycidylation of natural phenolic compounds. ... ). I'm really confused about the chemical...
10 July 2023 9,329 4 View
Hi, We are using Sartoflow Smart(Sartorius) to concentrate the retrovirus(RVs). RVs were produced in 10%FBS/high glucose DMEM and concentrated by using the 300 kDa of Sartocon Hydrosart...
10 July 2023 7,945 0 View
Hello! I am trying to determine the best controls for my western experiment. I was wondering what the best controls are with a small kDa size?
07 July 2023 566 3 View
I installed a superdex 75 pg myself, and the conductance baseline was flat at the beginning of the balance, and then the baseline was flat when the sample was eluted to 50%, and the last 50%...
07 July 2023 8,582 3 View
I am working on finalizing some of my data for a manuscript, and I have recently started plotting my RT-qPCR data as the delta delta-CT, or the fold change compared to an untreated control....
07 July 2023 9,773 0 View
I am trying to find the maximum acceptable limit of moisture content in powders for oral suspension, is there a reference that lists acceptable moisture content in pharmaceutical dosage forms on...
07 July 2023 5,646 5 View
What is the diversity of agriculture in India and why is agriculture diversification essential for sustainable development in Indian farmers?
05 July 2023 2,857 0 View
Hi I'm conducting research on the photocatalytic reduction of carbon dioxide, and I'm stuck at choosing the ideal column for my gas chromatography setup to quantify my results. The expected...
03 July 2023 5,049 1 View
I need a guidance from my research fellows in Bioinformatics here who are knowing on how to make miRNA sequences from RNA-seq data from NCBI with software such as Geneious Prime for instance as I...
03 July 2023 445 1 View
I am working on HUVEC cells in order to Establish the Oxidative Stress Injury Model Induced by H2O2. I want to treat the cells with 5mM H2O2 from 30%h2o2 stock solution for 16 hours. How to...
03 July 2023 770 4 View
I am currently working with a specific type of cells called mouse alveolar macrophages (MH-S) in my experiments. During cytometry analysis, I consistently observe two distinct populations when I...
03 July 2023 7,474 0 View
I did CAM assay recently. 0th day the vessels where intact and good. The drug incubation was of 48hours. After incubation, every eggs appeared different. Couldn't able to distinguish which is...
03 July 2023 9,286 1 View
03 July 2023 6,442 0 View
I am doing time resolved FTIR between a protein and CBD. My protein is at 66uM and the CBD at 1250uM - both in Hepes buffer (20mM hepes, 200mM NaCl pH 7). I tried a lower concentration of CBD in...
30 June 2023 3,575 1 View
I have been invited to join the editorial board of a journal called 'Arts & Communication', which appears on the website of ACCScience to be a newly launched publication. However, I have...
30 June 2023 3,714 17 View
I have a protein, when the salt concentration slowly drops to 300mM, there will be some precipitation, and the salt concentration eluted on the ion exchange column is also 300mM.
29 June 2023 3,988 0 View
Hello. I am trying to quantify intensity in neurons (w/mutation) compared to control. I chose integrated density but I noticed that mean intensity gives opposite results. I noticed higher...
27 June 2023 2,026 4 View
Hello, I dissolved polyethylene terephthalate in a phenol base solvent and sent it to electrospinning to prepare nanofibers, but the phenol freezes in the needle head of the syringe and blocks the...
26 June 2023 482 3 View
Hi there! I got amplification of my genes in the negative control in Real-time PCR experiment although the Ct values were above 30. I setup my reaction again after making new dilutions of PCR...
24 June 2023 4,231 9 View
While phosphoric acid is generally considered more stable than nitric acid, nitric acid is known to react with tri-calcium phosphate (Ca₃(PO₄)₂) and dissolve it
24 June 2023 7,053 0 View
If isolated mitochondria is stored in DPBS or PBS as opposed to a storage buffer with sucrose, does it affect the quality of isolated mitochondria?
23 June 2023 2,859 2 View
In relevance to drug delivery, the absorbance of EPIRUBCINE is measured at 480 nm in UV-Spectrophotmeter. Which would be the best selection between UV-Quartz and Glass cuvettes? Is any of this...
23 June 2023 4,948 2 View
Hello, I am planning on doing a TMT-labeled MS analysis experiment for global proteomics of glioma primary stem cells. Its my first time doing the experiment so Im referencing the protocols of my...
23 June 2023 9,214 0 View