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Questions related to Buffer
Hello all, I have transfected my cells with plasmid containing my shRNA sequences of my gene of interest and I'm looking this effect on 3 other genes, that when my gene is knockdown what happens...
09 January 2024 1,798 6 View
What is database replication? What does it do? What types of database replication are there?
08 January 2024 9,237 0 View
Hello there! I have a protein in my project that undergoes glycosylation. There are two main glycosylation products with different molecular weight. However, I do not have the glycosylation...
05 January 2024 1,211 0 View
I'm writing a report on enzyme activity and characterisation and i need help with understanding this: we were told to report on our results for a 1/10 diluted acid phosphatase. I want to know why...
05 January 2024 7,021 1 View
My research is based on hydroponics and soil-grown plants. The analytical analysis of plants is done through a similar methodology but I have too many results to be added in one paper. For...
04 January 2024 5,832 3 View
What are the important roles of microorganisms and role of microorganisms in composting and humus formation?
04 January 2024 8,467 0 View
Recently I tried to use my nanoparticles carrying GFP mRNA to transfect liver cells in vivo. And my plan is to figure out which kinds of liver cells (Like hepatocytes, Kupffer cells...) can be...
02 January 2024 3,204 5 View
I am comparing two different cell lines (LN229 and HA), LN229 as target sample and HA as reference sample. If I do the 2^-(ΔΔCt) method as LN229 - HA some genes get a fold change value of 41 and...
02 January 2024 9,223 2 View
Is there a formula for preparing of shutdown solution for flow cytometry. I found a formula as water (%99,79), 2-phenoxethanol (0,20%) and sodium benzoate (0,01%). But I am not sure that because...
02 January 2024 6,039 3 View
I want to deoxygenate oxyhemoglobin with nitrogen bubbling, but i can't observe the characteristic peak of deoxyHb in UV-Vis spectrum. Also, the concentration of my hemoglobin sample was little...
02 January 2024 5,653 0 View
What are the role of microbes in soil fertility and how microorganisms are important for maintaining of health soil and biodiversity?
02 January 2024 7,867 5 View
Hello everyone! The 2DEG density of the under-gate region of conventional depletion-mode GaN HEMT can be obtained through C-V measurement. However, a junction capacitor (Cj) due to the Schottky...
02 January 2024 5,613 0 View
I am a Msc student and my thesis is framed on developing a CNN-based approach to predict soil carbon hotspots using remote sensing data. Soil carbon hotspots are areas where the concentration of...
30 December 2023 3,997 1 View
For calculation of entropy change of a system (interaction between extracellular polymeric substances and heavy metals), it is essential to assume equilibrium mixing values. How to achieve this...
29 December 2023 2,492 4 View
At this stage can i store my sample?? Will protein be stabilize or for how much time it will be??
28 December 2023 5,119 1 View
Hi, everyone. I resently made the expression and purification of a GST recombinant protein. I am wondering whether this protein can have its function by added in the 293T or HeLa cell...
26 December 2023 10,118 2 View
What are the characteristics of integrated supply chain how can software help businesses better manage their supply chains?
25 December 2023 3,695 2 View
I am working on nanobody based purification system and in order to reuse it, I have to separate the nanobody from its ligand.
23 December 2023 433 2 View
I made a RNA extraction from mammalian cancer cell line and ı wanted to be sure whether there is a DNA contamination so I run my RNA on a gel. However, interestingly, I saw 3 bands. 2 of them are...
23 December 2023 7,405 2 View
I need a help regarding the molecular docking of a ligand which contains Ag or Fe as an atom, as I use Autodock vina and Autodock 1.5.7 and it doesn't contain their parameters. If someone knows...
22 December 2023 8,564 3 View
Hi all, We have been trying to figure out why we see such differences in our base pair (bp) fragment size between doing a 1-step PCR reaction vs a 2-step PCR reaction to prepare metabarcoding...
21 December 2023 7,806 0 View
Hi, We are facing a challenge of incomplete Titanium etch with H3PO4 (5min 70C). The cavities are partially opened in the 6-9 o'clock region near to the wafer edge on an 8" wafer. Can anyone help...
21 December 2023 7,316 0 View
I have bone marrow derived GM-CSF differentiated DCs pulsed with ova & stimulated with TLR ligand. Used these DCs to co-culture OT1 T-cells. After 3 days when I check T cell proliferation by...
20 December 2023 7,464 4 View
When I was acquiring half of my sample as live cells and half fixed, I used to get 30% positivity and now when Im taking the whole sample as live cells only, it increased to 90%. What is the...
20 December 2023 9,390 0 View