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Hello All, I am working on E. coli and want to study its gene expression profile using qPCR. I grew the culture till stationary phase and snap freeze the cell pellet using liquid nitrogen. I...
21 April 2023 7,269 4 View
My concentration was 123.5 ng/uL 260/280 Ratio - 1.725 260/230 Ratio - 0.401 Can I synthesize cDNA from the above RNA? Need suggestions please.
21 April 2023 7,061 4 View
I am currently running an HPLC to quantify the activity of my enzyme. I incubate it with my substrate which shows a peak corresponding to its product and also the substrate peak which wasnt...
20 April 2023 286 3 View
I am separating oil from oil-water mixtures. After separation what method can I follow to analyse the collected water to see if indeed the separation happened, method can be by GC/HPLC.
19 April 2023 3,041 3 View
Actually, I'm interested in evaluating the combined killing potential of various antibiotics. I would be grateful if someone could assist me by suggesting a straightforward program for evaluating...
18 April 2023 6,510 0 View
I have used to Qiagen powersoil pro kit to extract some DNA from bacteria, my first bunch of samples had good concentrations between 200-300 ng/ul on Qubit, and 300-400 ng/ul on nanodrop for...
18 April 2023 4,371 2 View
Most important differenc between xtt and mtt despite of cost effectiveness
18 April 2023 6,442 3 View
Hi all, I have been using PHA as positive control of stimulation on both healthy and diseased PBMCs for about a year. 2.5ug/mL, 10e5 cells/well. In the past few weeks, any cells I plate with PHA...
13 April 2023 5,435 0 View
We are dissociating deep pyramidal cells from 3-4 week old mice. We are using a Worthington Papain Dissociation System. We are wondering if there is another enzyme that would be more beneficial...
13 April 2023 6,528 0 View
When I gave the negative pressure to seal the membrane, I found that if I released negative pressure during sealing, the sealing resistance increases quickly to reach above 1 gigaohms. Is that...
11 April 2023 2,791 1 View
i am new to enzymology and want to study the discipline through history based books (i,e, how was the Kreb's cycle discovered, methods used then, etc). what are your suggestions to achieve...
08 April 2023 520 2 View
Dears collegues, I obtained strange derivative curves (difference between samples) of my PCR HRM : the peacks are angular. It's a 150bp amplification of ADN from a bacterial communinities with...
07 April 2023 2,289 0 View
It seems to me that everyone just refers to J. W. Goodman's book "Speckle phenomena in optics: Theory and Applications". I agree this is a good book, however in my opinion there are some...
06 April 2023 3,894 1 View
Hello, I am trying to figure out the issue with the isoform of pDNA isolated with GeneJETPlasmid Miniprep Kit and further digested with FastDigest enzyme in FastDigest Buffer (thermo...
06 April 2023 6,574 3 View
what is better BSA or Skim Milk for PVDF membrane blocking in western blots?
06 April 2023 3,138 4 View
i have used beta mercaptoethanol for protein extraction.. But now how to remove it from the sample extract so that i can go for freeze drying..
05 April 2023 579 0 View
I am having issues resolving PCR inhibition in rhino dung samples for a metagenome sequencing project. I used the DNeasy Blood and Tissue Kit to extract gDNA (with expected low yields, but DNA...
04 April 2023 9,201 3 View
Sodium Phosphate manufacture uses sodium dihydrogen phosphate and disodium hydrogen phosphate. PBS manufacture uses pottasium ohosphate monobasic, sodium phosphate dibasic and sodium chloride....
04 April 2023 1,029 2 View
I would like to ask a question. TMT labeled peptides by reacting with amino groups (primary amine groups), but the sulfhydryl-acetamide structure (carbamidomethyl of Cystein) formed after IAM...
01 April 2023 446 2 View
Which fungicide is used which increases the disease in plants more than before?
01 April 2023 548 1 View
I want to study the extraction performance of acidic extractant in different pH
31 March 2023 9,515 4 View
I want to know the exact mechanism
30 March 2023 6,707 0 View
Dear Colleagues, I wish to perform a genotype-phenotype correlation study in a family with a known mutation in RPGR-ORF15. However, the RPGR-ORF15 region is very tricky because it contains GC...
30 March 2023 3,883 2 View
I am trying to implement a synchronous buck converter as shown in the attached picture. When I connect a load to it and start raising the input voltage from 0V, I observe there is a spike in the...
30 March 2023 8,098 2 View