Contact experts in Buffer to get answers
2,092 views 3,987 posts
Questions related to Buffer
I have trouble interpreting the chromatogram because of some peaks with a long tail, I think that there is a problem with the column. Can you explain the temperatures you use to clean the column?...
28 September 2023 8,646 4 View
I found this paragraph on page 223, Chapter 10, Concepts of Genetics. "The phosphate group may be bonded to the C@2′, C@3′, or C@5′ atom of the sugar"
28 September 2023 7,080 0 View
Bovine serum albumin has a mass of about 66kDa. I want to do binding studies of Bovine serum albumin with several secondary metabolites with a mass up to 2.5kDa. Can you recommend a filter -...
27 September 2023 3,224 4 View
I am trying to do a phagocytosis assay with IgG opsonized beads and RAW cells, after the opsonization of beads I treat them with cells for 10-20 mins before they used to eat beads very...
27 September 2023 8,863 3 View
Hi there, I have qPCR results from 6 target genes and a housekeeping gene. Each different gene had its expression measured after 3 different treatment conditions and a non treatment control. I...
26 September 2023 2,598 2 View
Greetings to all. Currently, I have successfully purified the GST-tagged fusion protein. The protein degrades as soon as I remove the tag. It is an inherently disordered membrane protein. Please...
26 September 2023 6,814 1 View
What shows a good adsorption is more significant Adsorption capacity qe or Higher Percentage removal (%R)? How do these two affect each other? How can we say an adsorbent is best in terms of these...
24 September 2023 9,951 5 View
What are the ways to confirm the edited gene through CRISPR system?
23 September 2023 3,296 3 View
I've seen simulations showing this, but is there an intuitive way to understand this?
23 September 2023 2,766 0 View
I used sonication to disperse GO into water evenly. But when I added some hydrated metal chloride into above solution, GO will flocculate and settle. Hence, Will adding surfactant work? Or, what...
20 September 2023 363 0 View
Hi, I want to gentoype SOX10 mutants so that I can separate hets from wildtype fish. Are there well know or published primer sequences to use? thanks, Adam
18 September 2023 1,009 0 View
Can anyone who are culturing RL95-2 cells help me in this? The cells look very patchy, aggregated and even after 24 hrs very few have attached.
16 September 2023 8,958 1 View
We started to see such objects in the flasks containing our canser cell lines? What could these be? Could the cells have been contaminated?
15 September 2023 5,964 0 View
Hi, I’ve recently started having issues with a Zeiss axio cam HRm camera. The camera’s serial number and other informations are available below. The problem is that something is obscuring the...
14 September 2023 9,234 1 View
to obtain a better photocatalysis activity, does titanium dioxide need to be only anatase or a combination of anatase and rutile phases (my synthesis is anatase %77 and rutile %23)
13 September 2023 6,754 1 View
I need to know how to design,browse in lncRNA darta base and designing tools.
12 September 2023 4,289 0 View
I am trying to find a way to date calcium carbonate bioherms in Fayetteville Green Lake, to determine when the greatest periods of growth occurred. Traditional methods like C14 or uranium/lead...
11 September 2023 9,487 2 View
Hello Dear all! I would like to know if it is possible to have a stable cell line with a response element attached to a luciferase signal, also, Could it be possible to have in the same stable...
11 September 2023 2,675 2 View
I am doing a research on different Enrofloxacin-containing veterinary products (injectable and oral solutions)and observing the differences . some products are acidic and some are basic. So my...
11 September 2023 143 1 View
For example, a natural compound named ruin have IC50 value reported at 100microMolar concentration in HCT-116 cell line in one research paper, while the value differs to as low as 40microMolar in...
08 September 2023 2,544 3 View
good greeting I am from Syria I would like to continue my doctoral studies according to this research I want to cooperate in studying and university admission for distance learning I studied...
05 September 2023 4,506 0 View
I have a large no.of bee samples and thouight I can grind all the samples using miller and store at -80. How long can the lysed samples be stored at -80?
05 September 2023 2,768 0 View
Is humidity very high in the equatorial region and why is higher humidity found around the equator instead of around the poles?
03 September 2023 4,242 0 View
I have gold nanoparticles linked closely together by divalent ions such as Zn2+ or Cd2+ to form long gold nanochains. I was using APTES to form positively charged amine groups on the silica then...
02 September 2023 6,913 2 View