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Questions related to Buffer
I would like to unfold and refold mRNA in the absence of any enzymes. Is the thermal denaturation and cooling only way to unfold and refold mRNA? what is the safest temperature one could heat an...
16 February 2023 7,766 3 View
Hello friends, I have been carrying out cardiomyocyte isolations for a few weeks now and I want to get a homologous mixture (or at least 90%+ purity) of just cardiomyocytes to carry out some...
16 February 2023 4,321 4 View
hi i am working on long non coding RNAs i got the ct values around 30 for both diseased and control samples. what does it mean ?
16 February 2023 9,582 1 View
Hi, I try to purify protein expressed HEK293T I've tried many things, but I failed... * Binding: 0 mM Imidazole, Wash: 20 mM Imidazole, Elution: 500 mM imidazole * All buffer contain protease...
14 February 2023 3,806 5 View
I'm trying to amplify 8 targets in 1 tube by multiplex-PCR, but I can't get all the products by next generation sequencing. How to make it?
13 February 2023 3,696 4 View
samples are for refolding procedure
09 February 2023 3,588 4 View
Hello, I need to buy precast sigma milipore gradient gels (Bis-Tris precast gels mPAGE® 4-20%). Has anyone ever used them and could share their experience? Typically, I conducted protein...
09 February 2023 276 1 View
Hi everyone! I´m going to perform a conjugation between a single domain antibody (VHH from llama) and the HRP. I'm using a commercial kit from Thermo. The datasheet indicates an approx 4-fold...
08 February 2023 7,901 2 View
I am trying to do a docking between a ssDNA aptamer with an organic molecule using Autodock4. I have reviewed papers where they add Gasteiger charges to the aptamer but they don't mention anything...
08 February 2023 8,459 1 View
What is an easy method to count total leukocytes in rat blood? Can I just use a hemocytometer? Does anyone have any good images/protocols for what to look for? Do I need any special equipment? I...
06 February 2023 833 3 View
Looking for a cell surface marker to identify adult mouse cardiomyocytes (from the heart, not cultures) by flow cytometry???
03 February 2023 3,815 2 View
I am attempting to detect a number of proteins expressed by a bacterium during infection in a mouse model. However, the presence of mouse proteins in the serum and tissues is hindering the results...
03 February 2023 2,604 1 View
Does anyone have insight as to whether it's possible for K-internal to go bad? We are performing whole-cell patch-clamp recordings. For the past few months we have been having issues with cells...
02 February 2023 9,598 4 View
hi, I am trying to dissociate mouse hippocampus tissue into single cells with The Neural Tissue Dissociation Kits (130-092-628) and followed by cell debris removal, I was wondering how many alive...
01 February 2023 3,582 3 View
Hi, I am looking for recommendation of enzyme kinetics software that is able to fit/calculate initial steady-state rate of enzyme reaction. I have tried ICEKAT but also would like to compare it...
01 February 2023 5,369 4 View
I'm assessing the effect of different doses of various drugs on Breast cancer cell lines. All drugs were dissolved in DMSO. Using MTT assay, the impact of some drugs on proliferation was...
01 February 2023 3,003 5 View
I am currently doing Western Blots for adipose tissue lysates however I am having a hard time getting even loading especially when probing for loading controls (B-Actin and A-Tubulin). I feel like...
31 January 2023 6,986 0 View
We recently replaced the heating filament of our P-97 puller. Since then the programs have been producing variable pipette ends with same program. Every time the puller switches off we have to...
30 January 2023 4,514 2 View
I have a rare cell population and I am doing FACS sorting using cell surface marker staining to purify one cell type. I later plan on to pool the RNA from the same cell type from different...
28 January 2023 7,917 3 View
Hello everyone. Recently, I observe that my HEK293 cells in the 6 well plate have circular cells that are floating in the medium. This is only a small fraction of the whole cell maybe
28 January 2023 8,703 2 View
Hello everybody, I have to apply a Trizol protocol for the purification of RNA in viruses contained in culture media; in fact, it is the first time I use these techniques!!!! Our goal is to...
27 January 2023 9,546 3 View
Any answers will be helpful! Thank you so much!
27 January 2023 4,361 0 View
I am working on the hole transporting materials for photovoltaic, so i want to polymerised it electrochemically . is it possible to polymerised electrochemically the PTAA? Thanks in advance for...
26 January 2023 861 0 View
I have been trying to produce exosomes from platelets activated with thrombin. I usually get high yields after ultracentrifugation. However, the exosome pellets are very hard to resuspend and...
25 January 2023 4,698 2 View