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Questions related to Buffer
Why sodium hydroxide can cause increasing the fluorescence intensity for a fluorogenic reagent however acetone completely diminish fluorescence peak of the same reagent?
10 November 2023 3,791 1 View
Hi I am conducting a research in digital forensics where I need to identify key subjects (witnesses or perpetrators) of an incident. Therefore, I am in need of twitter event datasets to test my...
10 November 2023 3,658 3 View
We would like to obtain mitochondria from cancer cell lysates. However, we do not have a clear protocol. Additionally, we do not have the kit to enable mitochondria isolation. Do you have any...
07 November 2023 6,221 2 View
We use the salting out technique to precipitate wide variety of protein. How does NaCl only precipitates only collagen? Does NaOH pre-treatment help to remove Non-Collagenous Proteins (NCPs)...
07 November 2023 7,187 0 View
I want to digest sumo/His label when the protein in still hang on the Ni column. I wash the undesired proteins as usual, then balance the column with 3ml enzyme digestion buffer, then add sumo...
07 November 2023 9,932 3 View
I have been getting these long smeared lanes for very long in western blot. Every time I make fresh buffers and even I have changed the antibody brand. Nothing helped. Please provide suggestions...
06 November 2023 6,979 4 View
Hi, I need help processing for big data. I am trying figure out ''How does Twitter process big data? Also, what can be done with big data?'' Can you explain briefly and/or can you suggest...
06 November 2023 9,268 3 View
Kindly mention the types of microRNA.
06 November 2023 7,227 1 View
I had an issue with the results concerning a bottom-up experiment using tissues preserved in FFPE. The FFPE samples (1 kidney and 4 thymus tissues) were de-paraffinized by placing them in...
06 November 2023 7,004 1 View
I need the mapping the breast cancer patient journey in a process map format
05 November 2023 2,714 2 View
I have the human microglia cell line (HMC3), they are cultured in EMEM medium supplemented with 10% FBS and antibiotics (100 IU/ml of penicillin and 100 μg/ml of streptomycin). I'm planning to use...
01 November 2023 5,520 4 View
Hello everyone, we are trying to generate stable cell line expressing two proteins of interest on the membrane using CRISPR. I am new to this technique and not completely sure about the protocol...
01 November 2023 8,071 0 View
Can anyone recommend a database of PW or CW Doppler ultrasound (preferably with images)? I need to do some learning on Doppler imaging. Thanks a lot!
01 November 2023 3,441 0 View
You can add whatsapp or facebook.
31 October 2023 4,241 0 View
Pd2(dba)3, P(o-tol)3, Toulene, K2CO3.aq
30 October 2023 5,212 0 View
Dilution can not be enough I think, because I'm not sure whether it is obtained accurate results or not that colloidal particules exist in the medium.
30 October 2023 3,964 3 View
"For a mixed-integer linear programming model M with feasible solutions, by taking the non-zero valued variables from the linear relaxation solution of model M to construct the solution space of...
30 October 2023 6,111 3 View
I have been trying to expand NK cells isolated from Healthy Donors in CTS NK Xpander Medium from Thermofisher with IL2 at 500U/mL conc. being provided. Alongside, I am pre-activating NK cells with...
30 October 2023 9,253 4 View
Can I store RNA in isopropanol for a long time at -20 °C?
29 October 2023 3,145 2 View
Nanoluciferase itself is not typically secreted from cells naturally, but I don't know why I detected it in 293T cell supernatant. Is there any method to inhibit the secretion?
28 October 2023 9,603 0 View
I am currently doing an experiment related to nanoplastics. Recently, I did an immunostaining experiment with triton X-100 to permeabilize the cells, I found out that the nanoplastics signal...
27 October 2023 7,295 0 View
Hello everybody. I'm trying to study HPHT synthetic diamond plates with low (first nm roughness). When i taking 100×100 μm photo by AFM, you can see a lot of flakes/dirt on them. The question is...
26 October 2023 8,019 4 View
Why does a solution of 50mM Tris and 20mM CaCl2 sometimes precipitate? This buffer is used for protein purification.
25 October 2023 1,963 1 View
I am currently doing undergraduate work, specifically the methodology part. I want to be able to create distribution models, however I do not know how I could create one based on the distribution...
24 October 2023 3,806 3 View