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Clone the sequence of a protein in two different vectors, one is pET21a+ which gives a His sequence at the C-terminus and the other vector is pET41A+ which gives His at the C-terminus as well as...
20 June 2023 9,153 1 View
can you plese suggest other ways of restriction digestion of DNA.
20 June 2023 7,890 3 View
I am looking for a protocol which I can get the purified DNA from FTA cards easily and inexpensively. Then I want to use TE buffer instead of using buffer AT, ATL. The thing I want to know is...
16 June 2023 5,862 2 View
Hi everyone! I'm wondering if anyone here has any experience with the Dynabeads Protein G immunoprecipitation kit (#10007D) from Invitrogen. I'm working on a project that involves...
14 June 2023 4,957 5 View
This question addresses the practical considerations for optimizing CRISPR-mediated knock-in experiments in mammalian cells. It explores aspects like donor DNA design, homology arm length,...
14 June 2023 7,658 0 View
Why transfer of heat takes place faster in liquids than in solids and what is the process where heat flows in the absence of any medium?
12 June 2023 2,233 4 View
Hi dear all. I am testing the response of antibodies to sheep red blood cells during four experimental stages and I Intend to compare the four stages using a glm model. Since the response variable...
12 June 2023 9,174 0 View
Hi, We will like to receive advice to improve RNA quality and quantity. After many trials and calibration, we get a protocol that gives better RNA quality and enough quantity for downstream...
11 June 2023 8,068 4 View
Dear all, I am conducting research on site-directed mutagenesis of crystalline proteins. My aim is to verify the desired mutation in the protein sequence using the MALDI-TOF mass spectrometry...
08 June 2023 918 6 View
Hello, In our lab we produce a protein recombinantly using CHO-K1 cells. Therefore, these are first grown in adherent culture, then adapted to 200 mL suspension, after 2 days expanded to 400 mL...
08 June 2023 1,681 0 View
It's my first time doing Annexin V Assay There was no problem until the sample preparation, but when I went to take FACS and put 200uL of PBS, the cell seemed to be aggregated. Protocol The A549...
08 June 2023 785 0 View
I want to do MALDI-TOF mass for my protein. For its procedure I need to do bis tris electrophoresis of immunoprecipitated protein, then excise protein band. For running bis tris electrophoresis, I...
08 June 2023 851 5 View
I have frozen my antibodies dissolved in gelatin (the instruction says do not freeze), is it still possible or right for me to thaw it ?
06 June 2023 8,716 1 View
Either It will be Sodium Tetraborate decahydrate or sodium tetraborate or Boric Acid with NAOH or I will use NAOH to adjust pH)?
01 June 2023 4,637 1 View
Hi there, I want to ask whether a ratio of 260/230 of 1,55 is considered low for primer after its resuspension. Thanks in advance
31 May 2023 1,659 3 View
Given the completion of mechanical testing (e.g., compression, pull-out, etc.), this inquiry questions whether a cytotoxicity test using the MTT assay alone is adequate for assessing material...
31 May 2023 2,989 2 View
I found a poster presentation of a FACS-based Smac/DIABLO release assay, which would be a good approach for leukemic cells, but I can't find the protocol. Does anyone have it? Another approach...
30 May 2023 6,415 0 View
Hi all, I am preparing citral loaded chitosan nanoparticles using the ionic gelation method. I want to know which one out of 1N NaOH and 0.1M NaOH can be used to adjust the pH of my chitosan solution?
29 May 2023 5,896 3 View
Hello there, I would like to correlate the mRNA expression of a gene obtained from patient blood samples with surrogate parameters like HbA1c and data like body weight, age, etc. However, I am...
28 May 2023 2,699 2 View
Dear all, I have a superdex 75 Increase 10/300GL. I have attached this column to the FPLC several times but today i got a error for high pressure and the FPLC shuts down. I noticed some leakage at...
25 May 2023 3,180 2 View
Hi everyone, I was wondering if someone has had any experience purifying DNA at lower g or rpm values than indicated in a comercial minicolumn protocol. I'm using a Qiagen QIAamp DNA Mini Kit and...
24 May 2023 755 0 View
I worked on a colorimetric assay with TMB and nanocluster and H2O2. the blue color is observed but after around 2 h, the blue color disappeared. Does anyone know the reason for this? who has such...
22 May 2023 9,312 3 View
Hi all, Hope you are well. I was wondering if someone could point me in the right direction of methods for protein knock in on a primary human cardiomyocyte cell line? I am not sure what would be...
19 May 2023 5,657 1 View
I am trying to neutralize nitric acid since the pH of my solution drops to zero after nitric acid treatment of my sample.
19 May 2023 6,374 3 View