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I have already tried different combinations of optimized DNA Nicking Assay protocols. The plasmid DNA that I use is puc19 from a transformed E.coli. Every single time I perform my Assay, I always...
31 May 2024 1,637 2 View
Hi all-- I'm preparing plant root samples for RNAseq and have been investigating some samples that had suspicious bands in their lanes when I ran a 1% non-denaturing RNA QC gel. To spot check...
27 May 2024 7,288 1 View
How is crop diversification helpful to stabilize the yield of the crop in the system and important aspects of diversification of agriculture activity?
26 May 2024 9,079 3 View
Why does India need climate resilient agriculture systems and how does climate change affect agricultural production and sustainable mitigation?
24 May 2024 589 3 View
How to establish the calibration curve of PNP in relation to concentrations in a microplate and for measuring the activity of lipase, what do we use? Substrate stock: 20 mM p-NP palmitate in...
20 May 2024 1,415 0 View
I have 2 proteins-one is 19KD and other is 5KD. I tried detecting both of them through western blot using 20% SDS gel. I transferred them into 0.45 PDVF membrane for 100mins, I could detect the...
20 May 2024 3,129 0 View
We live on land and we cultivate, install our cities and industry on this land and we pump water. However, the lack knowledge concerning soil, subsurface lithology and water resource from...
17 May 2024 7,008 2 View
What selectable marker genes do you suggest instead of hygromycin in the gene knockout project in rice?
15 May 2024 2,796 0 View
Hello, I hope that if you are reading this you are doing an interesting science :) I want to design guide RNAs for CRISPR Cas9 knockout screen I'm planning (for human cells cultures and mouse...
05 May 2024 4,809 1 View
Do you require Aerosol suction in every procedure apart from dental prophylaxis? If so what is the ratio of this per dental section, let us say if you have 4 dentists, would one Centralizes...
25 April 2024 8,822 0 View
Both concepts have deep roots in human cognitions.
18 April 2024 9,481 1 View
Uploading of Research Publication
17 April 2024 6,716 2 View
Dear Prof, Greetings ..... I'm Sebastian, would like to do research on paper publication and its impact in the ResearchGate database, like Web of Science and Scopus. So if you all have any idea...
16 April 2024 7,319 1 View
" Back Off! I just backed up topol_Other_chain_A2.itp to ./#topol_Other_chain_A2.itp.2# Making bonds... atom HA is missing in residue HEM 600 in the pdb file atom HA is missing in residue HEM...
13 April 2024 2,562 0 View
This is to understand on how the physicians or microbiologists and other related health workers respond to such unprecedented situations.
13 April 2024 8,025 3 View
In 1980, Fred Miles proposed (contrary to the views of Masao Ito 1972) that the purpose of the cerebellum is to house efference-copy representations of all movements being commanded by the...
12 April 2024 1,787 0 View
I'm currently facing difficulties in conducting my experiment, which aims to investigate the MMC 5-level output voltage using a DSP controller and MATLAB ePWM signals. I would appreciate your...
10 April 2024 8,063 0 View
Does the presence or absence of crystalline water affect the heat of dissolution? For example, Is the dissolution heat of anhydrous CaCl2 and Calcium chloride dihydrate (CaCl22H2O) the same?...
08 April 2024 771 0 View
Relative molar composition of 1.0 TEOS : 0.2 CTACl : 0.0026–0.017 LS277 dye : 10.4 TEA : 142 H2O was used.
05 April 2024 6,876 1 View
Can frogs transmit blood parasites to humans? If yes, how? And if not, why?
04 April 2024 6,401 0 View
My mitsubishi P93D thermal printer has become landfill material because ther are no drivers available to support it. I have a Corning Axygen Gel Doc cabinet. I woudl reall yliek tohave ahtermal...
03 April 2024 8,490 1 View
Do anybody have information about customized chemical synthesis (Org. comp.) service provider (Paid service) for compounds at laboratory level?
30 March 2024 9,705 0 View
I've selected protease as the optimal enzyme for eliminating gluten formed from flour. Could you please provide insights on the best enzyme for removing gluten, dosing methods, and how to identify...
25 March 2024 1,702 2 View
For DNA islolation
14 March 2024 7,747 4 View