I have trouble interpreting the chromatogram because of some peaks with a long tail, I think that there is a problem with the column. Can you explain the temperatures you use to clean the column? Did you compare any temperatures to clean it effectively?
You could try to slowly hire up your chromatograph and then leave it all the night at the maximum temperature allowed (10ºC lower than the max one tolerated by the column).
Thank you so much for your answers, I wrote down the steps you recommended. I hope it will work.
Its a good practice to include a cleanup procedure with your method when working with sediments or samples that are not relatively clean.
You can raise progressively the temperature from 30 to 30 degrees waiting some minutes on each temperature to you achieve the maximum temperature of you column. Then you keep that temperature for a long time.
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