The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
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Hi, I am doing a mixed lymphocyte reaction with isolated PBMCs. To assess proliferation I have tried using CFSE, however, I can only see one peak after day 5 and not multiple. Has anyone faced a...
27 March 2024 4,889 1 View
That is, how many milliliters are used in each surgery? I have searched for information regarding consumption by respiratory volume or per kg of weight but I cannot find information.
25 March 2024 6,706 1 View
Hello everyone, I am seeking guidance on the analysis of scanned films obtained from Proteome Profiler Mouse Cytokine Array Kits, specifically focusing on the quantification of dot intensity using...
24 March 2024 1,293 2 View
Dear All, I am encountering challenges with AAV-mediated neuronal transduction, where high volumes lead to significant cell death, while low volumes yield inadequate signal levels. Here's a brief...
23 March 2024 4,321 5 View
Image processing software
21 March 2024 2,009 0 View
I am currently working on my thesis research on purchase intention. My reference article is Brazilian consumer perceptions towards second-hand clothes regarding Covid-19 by Amaral & Spers (2022),...
20 March 2024 7,425 0 View
Stimuli norming prerequisite of plausibility test is the issue in nonnative context of research. Regards
20 March 2024 8,437 0 View
My student optimizes the RNA dot-blot method using a biotinylated cDNA probe. The aim is to detect a positive hybridization signal of a probe complementary to transcripts of a housekeeping gene...
18 March 2024 4,687 3 View
Hi all I need to keep some samples at -35 to -25 degrees on an orbital shaker for a few hours or so. The only way I can think of doing this is to keep the samples a land shaker in a freezer....
18 March 2024 6,181 1 View
I am producing lentivirus for my masters thesis and I do not have access to an ultracentrifuge- I have been looking for a protocol to concentrate my samples using the PEG method but there is many...
18 March 2024 2,143 2 View
The cell wall is a rigid structure that provides protection to the cell that contains it. Molecules that need to enter or leave the cell must cross the cell wall in addition to a plasma membrane....
17 March 2024 7,143 0 View
Hi everyone Is there a relationship between standing long jump performance and maximum knee flexion angle at the moment of take-off? Does a higher maximum knee flexion angle indicate a better jump...
16 March 2024 6,544 0 View
Hello all, Currently I am working with eye-movement data collected using the Tobii Pro Glasses 3. We have a clinical group and a healthy control group who performed a series of tasks while...
15 March 2024 8,380 2 View
Hello everybody, I'm currently performing Western blot and I am a beginners for Western blot, so I want to ask that Can I replace blotting paper by any other papers?
15 March 2024 1,824 3 View
Does anyone know any protocol I can follow for muscle fiber-type staining for paraffin-embedded tissue?
14 March 2024 621 2 View
Why is it important to preserve genetic diversity as well as species diversity and biological significance of genetic diversity between populations?
13 March 2024 6,564 2 View
I used Natural Goat Serum (NGS) for my immunostaining protocol. However I noticed that NGS is also combined with Bovine Serum Albumine (BSA). Is there any advantage to use both instead of NGS...
12 March 2024 4,692 2 View
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12 March 2024 2,922 2 View
what are the indices of nutrient availability?
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The motivational machinery of the brain in primates (composed of the anterior cingulate cortex, hypothalamus, amygdala, periaqueductal grey, lateral reticular formation, and parabrachial nucleus)...
11 March 2024 7,807 0 View
system xC- exchanges intracellular glutamate within the astrocyte with extracellular cysteine. Are there any studies that suggest this could affect calcium signaling within the astrocyte or ATP...
10 March 2024 1,167 0 View
What is the importance of recycling nitrogen and carbon in an ecosystem and organisms are responsible for cycling energy through an ecosystem?
09 March 2024 7,952 1 View
I'm preconditioning MSC in hypoxia and want to perform a wound-healing assay. For that, I need to take images every "x time". How can I do the images without interrupting hypoxia condition? Thanks
08 March 2024 7,417 2 View