The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
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Questions related to Brain
Hello everyone! I´m recently working in a biochemistry-lab as a medicine student and I´m doing immunostaining on mice brain´s. However, the slices I´m treating will look kinda good in the first...
08 June 2021 2,614 5 View
DREADDs will be used in rodent
08 June 2021 9,832 3 View
Hi, I am looking for a dataset including paired low and high quality MR images of brain (healthy subject). It would be great if the dataset includes multi contrasts such as T2-weighted, flair,...
07 June 2021 8,267 1 View
I want single-cell RNA seq data for adult human brain. Can anyone help me with a dataset?
06 June 2021 9,686 3 View
We did a primary culture of the hemispheres of a mouse, but it is possible that within the culture there are fibroblasts, so how can one distinguish between astrocytes and fibroblasts,...
04 June 2021 9,074 3 View
I would kindly request an expert input on the platforms or tools such as BioEdit but which are specifically designed for the visualization and sequence alignment in Methylation...
03 June 2021 7,260 3 View
I want to find a good dye to verify cannula placement in SD rat brain following stereotaxic cannula placement. Currently, I am trying Chicago Sky Blue (1% ) in saline
02 June 2021 1,070 2 View
why do not we start considering the death as the brain one not the cardiac ?
30 May 2021 5,219 7 View
Hi all, I am using several muscarinic receptor antagonists at comparable doses in my study, and I wish to compare their efficacies across experiments. To add more, experiments are performed in...
30 May 2021 9,530 3 View
I am having a bit of difficulty understanding the cell seeding process and concepts, so as a result, I am having difficulty understanding how many flasks of cells I would need to passage each week...
30 May 2021 2,440 2 View
Dear all, I am doing WBs for the evaluation of Neurogenin-3 protein levels in mice brain lysates using a polyclonal antibody from Merck and I have seen that it shows multiple bands instead of one...
28 May 2021 9,968 1 View
Dear experts in local field potentials, I am recording local field potentials from living mice and encounter two issues. I use glass pipettes as recording electrodes and the diameter of the...
27 May 2021 1,257 3 View
Hi, I have always embedded my mouse brains (PFA fixed followed by a 30% sucrose bath) in cryomolds with OCT (Tissue-Tek) before freezing and cutting with a cryostat. My colleagues however flash...
27 May 2021 9,636 1 View
My proposal of a scientific theory of Sentience - to be called Sentiomics - gives a systemic explanation of this phenomenon. The cold sensation is not a reflex of the physiological state of the...
23 May 2021 2,661 25 View
I am planning to do staining on mouse kidney, but currently I am not able to process the tissue using paraffin hence I want to freeze the tissue. I have done this with brain, at which I placed the...
16 May 2021 1,605 3 View
Hello everyone, I am working to isolate/immunoprecipitate homologs of a protein (RdR) from an invertebrate. There are no commercially available antibody for any of the homologs for my organism....
15 May 2021 8,091 3 View
Suppose we have two resting state EEG data sets recorded with different recording setups: one of them with a 256-channel setup and the other with a 27-channel setup. Both datasets are recorded...
14 May 2021 2,300 4 View
Hello! I am trying to figure out how to run an analysis for a longitudinal moderated mediation. I will be collecting data twice from the same individuals in a mediation study while employing a...
13 May 2021 5,354 3 View
I would like to stain living THP-1 macrophages with concanavalin A (texas red) and observe them under the fluorescence microscope. The idea behind this is that we want to observe the uptake of...
12 May 2021 7,689 4 View
Hello everyone! Is there any tutorial to calculate internal consistency (Cronbach alpha) and split-half reliability (spearman brown formula) of resting state EEG data? Preliminary data reduction...
08 May 2021 4,683 2 View
I have different readings of culture using PNPP at 405 nm. how can I know the concentration of the protein depending on these reading
06 May 2021 9,863 4 View
We want to create a PR and communication campaign that the government will use to motivate the public to use public transport instead of privately owned cars in peoples´ daily lives. We want to...
03 May 2021 7,445 15 View
histopathological, histochemistry, a guideline for brain tissue processing.
30 April 2021 7,333 2 View
We are doing calcium uptake experiments. For the liver and other organs, the preparation seems to be good but facing problems for brain mitochondria isolation. Is there any other method to isolate...
29 April 2021 3,264 3 View