The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
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Questions related to Brain
We are interested in the quantification of RNA from zika virus in the brain of embryos. We will use the RNeasy Lipid Tissue kit for extraction.
01 October 2021 7,959 0 View
Hi, The ITC protein (73kda) with ligand (GTP) showed no saturation pattern in the ITC experiment even after changing both concentrations. The pattern remains the same in the repeated experiments....
30 September 2021 6,598 7 View
Hello, I have used Sudan Black B for quenching autofluorescence in brain slices and it worked very well (0,1% SBB in 70% EtOH). I am now performing immunofluorescent staining on PFA-fixed,...
20 September 2021 343 5 View
Hi everyone, Does anyone know why the second sample from the left look small and circular and not linear like other samples? The problem is that after imaging they also look like that. All bands...
17 September 2021 1,568 3 View
Good afternoon TBSS experts, I am in the process of performing quality assurance on a set of diffusion imaging data on which I plan to run a series of TBSS analyses. As I did not collect this...
13 September 2021 7,813 2 View
Anyone know how to measure catalase activity in brain homogenates in terms of μmol/min/g of brain. Reaction was stopped by addition of dichromate and absorbance was measures after 1 min at 570 nm....
10 September 2021 6,181 1 View
I've read several works that assess catalase assay from samples by spectrophotometry based on Aebi (1984). Based on this, some works employ a microplate for this determination, and this is what I...
10 September 2021 662 4 View
I want to work on texture analysis of pituitary and stalk tumors based on MRI and other imaging. Can anyone suggest recommended software like image J etc. Regards
06 September 2021 1,689 3 View
Gentlemen, We are preparing a project to access a grant, but we are missing a lab that can help in the first phase of Proof of Concept and Safety. The lab should be European (But not spanish,...
05 September 2021 3,014 3 View
Hi everyone, I am trying to measure calcium sparks and transients in freshly isolated rat mesenteric smooth muscle cells using Fluo-4 (5 microM). we have a Zeiss LSM 900 confocal microscope that...
02 September 2021 7,395 7 View
I would like to calculate the free energy using MMPBSA in different segments of the trajectory. These segments correspond, mostly, to the dominant conformers. Considering that these segments...
27 August 2021 4,127 3 View
Dear all, I am looking for some help to understand the nature of PPI analysis better. Is it possible to get different measures of connectivity when looking at the same two brain region and...
26 August 2021 6,106 1 View
How to done current distribution plot in CST software for patch or slot antenna?
25 August 2021 2,569 3 View
Any Journal for surgical case Report which is economical?
23 August 2021 979 6 View
Hi there, I am working with an EEG dataset that looked at alpha, beta and theta frequency range in 4 brain regions: frontal, parietal, temporal and occipital. Participants performed two tasks:...
17 August 2021 556 3 View
Hi there, how do you isolate human myeloid derived suppressor cells (MDSC) from PBMC by MACS? Kits are available for murine cells but for human cells only FACS seems to be completely established...
15 August 2021 5,615 2 View
Good day scholars. Please can anyone help with detailed protocol(s) for the determination of Beta-Amyloid, dopamine, Nitric oxide (NO), Monoamine oxygenase A (MAO-A), and Acetylcholine esterase...
09 August 2021 2,325 1 View
Any tips, observations, suggestions are appreciated about stereotaxic surgery involving cannula implantation.
06 August 2021 6,328 0 View
Hi, I was hoping those working with organotypic or acute brain slices might be able to point me in the right direction of the equipment needed to aerate aCSF or brain cutting solution. I am...
05 August 2021 5,543 2 View
My colleagues and I have raised many issues related to the misuse of statistics. We have examined two meta-analytic studies and found the majority of the individual studies failed to be fraught...
05 August 2021 9,452 13 View
In my project, I need to perfuse the brain for better IHC results. We first perfuse the animal (mouse) with PBS, then 4% PFA in PBS; both solutions were kept at 4 degrees and on ice during the...
05 August 2021 9,603 2 View
I have a resolubilisation problem with serum samples. 250µl serum was 500µl lysis buffer (8M Urea, 100mM TrisBase, 2% w/v SDS) and centrifuged at 16.000 x g for centrifuged at 16,000 x g for 5min....
04 August 2021 3,629 4 View
Hi everyone, would anyone have any advice on how to input and analyse true/false questions on spss. I have 30 statements, 15 are neuro myths and 15 are brain based knowledge questions and...
01 August 2021 2,864 2 View
Hi All, I have recently started working on neural networks for hardware implementation where we normally work with fully connected networks for pattern classification. I am facing...
30 July 2021 117 7 View