The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
1,195 views 2,696 posts
Questions related to Brain
kindly, provide link if any thank you.
25 December 2021 3,752 3 View
Hello everyone I want to activate mice B cells with CD40 and IgM to express OX40L. but I don't know the quantity of cells which needed for CD40 and IgM. I was wondering if you have a good...
24 December 2021 2,826 4 View
I have a molecule with 5 secondary amine as a functional group and I need to do a reaction on one of them. How can I protect the other 4 secondary amine groups?
16 December 2021 4,484 3 View
I encountered some problems during the western blotting of protein extracted from zebrafish larvae. I use the chloroform: methanol=1:3 configuration to remove the yolk solution, but after...
16 December 2021 236 2 View
I have a molecule with 5 tert-amines and need to protect 4 amine groups.
15 December 2021 3,019 2 View
Hi everyone! I'm trying to change the beam theory: I usually utilize the beam188 or beam189 element type in my simulations, and I discovered on the internet that this element type is based on the...
15 December 2021 4,510 6 View
Hello all! I'm in need of some help. I'm currently trying to conduct a Western blot using Ki-67 (359kD) and want to know if using the semi-dry transfer method would work on this protein? I have...
02 December 2021 1,962 4 View
Recently, I did MD simulations using online tools. But I am not able to calculate the ligand binding energy of my complexes. So, how I can calculate the ligand binding energy of a complex by using...
29 November 2021 4,780 4 View
My p.21 mice have started developing seizures after intracranial injection of 4ul AAV to the left lateral ventricle. I’ve done ~22 of these procedures already with no issues. I’m using a 24G...
15 November 2021 6,691 1 View
Hi All, I pelleted mixed skeletal muscle mitochondria from C57BL/6 mice using 600g and 10,000g spin. I resuspended the mitochondrial pellet in 200 uL of 1.167 M KCL and 58 mM Tris + 66 uL 16% SDS...
13 November 2021 9,191 2 View
Hi I plan to produce lentivirus for my gene of interest, I've not done this before and would like some advice please. I plan to buy pLJM1 and cut in my gene of interest. Does anybody have a...
12 November 2021 2,670 1 View
I want to use DiI (or other dyes that work in postmortem tissue) to trace inputs to the striatum in adult mouse brains (mainly cortico-striatal and thalamo-striatal circuits). The brains have been...
11 November 2021 1,247 2 View
Hi all, I am trying to do a migration assay with 2 cell lines: MCF-10A and 4T1, however, when trying to create the wounds they do not form cleanly as they have patches or lines of cells in the...
10 November 2021 8,405 1 View
Is FcR blocking reagent necessary for sorting epithelial cells by using CD326 microbeads and MACS? Or does it make the specificity better comparing to not using?
09 November 2021 5,627 1 View
Maybe you have some ideas about where this backround is coming from? I am not sure if this comes from blotting, blocking or if this is dirt? Thank you very much in advance!
09 November 2021 8,629 3 View
Hello everybody, I observed smeared band of target protein (97 KDa) in western blot. Other proteins like beta-actin, AKT seemed normal and clear. Hers is other information: 1- The cell lysate was...
02 November 2021 3,984 3 View
So I have a data set where I have 5 repeated measures of a variable, but also need to check for interactions with gender (categorical), and a continuous variable (let's say minutes of something)....
01 November 2021 7,954 3 View
I already used 30% sacarose to preserve brain tissue for cut in cryostat, but I don't know if I can do the same for agar inclusion instead of OCT inclusion.
28 October 2021 9,934 0 View
Tricky or stupid question : I measure in different cell lines Ca-oscillation upon stimuli over a period of 4-6min. I use FLuo-4-AM dye from Thermo Fisher, btw 3 and 4 microMolar. Incubation in...
26 October 2021 3,444 0 View
I want to know at what dilution, thanks!
25 October 2021 1,668 0 View
I want to culture a specific bacteria on Nutrient Glucose Agar..so need to inhibit or suppress the fungal growth contaminations on that medium... please suggest me which is the best antifungal...
14 October 2021 5,652 8 View
Hi, good day...I conducted a human study toward the end of 2019, and I have kept my samples at -80 degrees since. Now I would like to analyse the expression of transcription factors (Nf - kB and...
14 October 2021 4,914 4 View
Dear experts, I am new to fMRI and I am working with rat fMRI data. I am performing the preprocessing steps in fsl (FMRIB Software Library) and since it designed for human brain, I must scale up...
13 October 2021 7,505 5 View
The BCI system does not have a particular application, it's just based on working with cognitive tasks. But there's doubt in what tasks to use and why
12 October 2021 7,850 2 View