The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
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Questions related to Brain
I have seen different results published online using CATALASE. I know that the outcome of the analysis depends on the type of tissue, and protocol used but I have seen gotten different results...
29 July 2021 6,095 4 View
Hi everyone, Does anyone know why these blots look like this? I have been dealing with this issue for a few month now. This effect is very clear on smaller proteins (basically anything below...
25 July 2021 2,033 8 View
I know some, like CUT&TAG, atac-seq, mass spectrometry etc. But would appreciated knowing others
24 July 2021 5,293 2 View
Hello, I want to obtain RNA from hypothalamus of rat. I planed protocol that I sliced brain into 100um by cryostat (-10℃) and punched out hypothlamus with brain puncher. However, I couldn't slice...
22 July 2021 4,270 11 View
Hello! I am looking for the working protocol, which I could use for clearing the brain section, 105 µm thick (hemisphere). Without this I am not able to reach deeper into the tissue than 20...
14 July 2021 6,307 2 View
There are lots of distressed co authors out there who were promised equal credit but didn't get it in the end, and some were promised 2nd co author but found themselves listed as 3rd or even 4th....
14 July 2021 9,683 3 View
I have a stock solution of 20 uL which contains 4000 ng of cDNA. My desired reaction for each PCR is 20 uL reaction and I need a total of 8 uL containing 10 ng of cDNA from my stock solution....
14 July 2021 2,542 2 View
I am currently studying Rat Brain in Stereotaxic Coordinates (Paxinos and Watson) and I have been looking for research studies in which laboratory measurements (such as chemical response,...
08 July 2021 8,031 3 View
Hi, currently I am trying to isolate nuclei using the "10xGenomics Sample Preparation Demonstrated Protocol". The protocol suggested varying the lysis time to see which one is the most optimal and...
06 July 2021 9,767 3 View
Hi From experience does anyone know if there is a link between cell passage number and gene-editing efficiency? I suspect there might be an inverse relationship (ie high passage number = lower...
05 July 2021 4,725 2 View
I am currently working on different human solid cancer cell lines such as Huh-7, HepG-2, A549, MCF-7, Caco-2, and HCT-116. And I want to use the confocal and fluorescence microscope to detect...
04 July 2021 4,261 3 View
why all spectra of metal complexes are shifted to a lower wavenumber and reduced in intensity? By examining infrared (FT-IR) , does the opposite happen, that the shift is towards the highest...
30 June 2021 2,295 6 View
Hi guys, I have technical difficulty with Micro-CT. My instrument model is BRUKER Skyscan 1275. When I use my brain tissue to scan, I cannot distinguish brain parts. My voltage is 60Kev and my...
24 June 2021 9,132 2 View
we want to find the best miRNA gene to normalize our data in qPCR. specially in Rat brain tissue.
24 June 2021 7,959 2 View
I tried to estimate the correlation coefficient of AOD with water precipitation, and AOD with visibility for a short period of time. I found that for a long term data set, researchers have also...
24 June 2021 6,837 6 View
Aside from embedding brains in an OCT block, are there other ways to section multiple brains at once?
19 June 2021 3,993 4 View
Hello Everyone, I use tamoxifen inducible cre (cre/ERT2) system to knockout a gene at embryonic day (E11.5). For that I am injecting 1mg 4-hydroxytamoxifen intraperitonealy in 100ul corn oil at...
17 June 2021 4,444 3 View
Hi, I am looking for an 8 channels EEG system. I need suggestions. I found a couple of websites. I would appreciate it if you share any of your experiences regarding the ones below or share any...
16 June 2021 5,168 3 View
The brain tissue sample is stained with NeuN. I want to be about to automatically count neurons especially of the dense area of the DG region of the hippocampus.
15 June 2021 9,658 3 View
There are a large number of articles using EEG and fMRI to study the evaluation and prediction of upper limb function of subjects after stroke, and there is a lack of research on lower limb...
15 June 2021 3,182 5 View
Turbulence model for blood flow simulation
15 June 2021 5,598 12 View
I was hoping someone may know if there is a method whereby a marker could be added to P. gingivalis and then if the bacteria were to be inoculated into a C57BL/6J mouse it would be possible to...
10 June 2021 6,411 4 View
Hi all, I'm having some trouble with immunostaining: streaky-looking DAPI. It's not totally consistent across a given slide (8-well chamber slides), and the degree of mis-staining is variable...
10 June 2021 9,876 5 View
Hi, is there anybody who can share a good protocol for cell membrane (transmembrane) protein extraction from zebrafish brain tissue to be assayed by Western.? I tried one with a soft lysis...
09 June 2021 8,812 3 View