The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
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I click on "verify your email. I add my institution's email. But it is repeatedly rejected saying: "too many uncofirmed verifications have been sent"!! I go to my email and do not find any...
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Dear colleagues, I am seeking advice on troubleshooting a challenging in vitro supercoiling assay. My goal is to demonstrate the histone chaperone activity of a recombinant protein by observing...
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In a current study, I have utilized the UPLC-MS-MS to identify the phytochemical components of a plant extract. Using the aid of a database, a group of phytochemicals was suggested to exist. In...
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It's an uncharacterized protein, and before going for protein expression and purification, we want to know the subcellular localization of the protein for gaining better understanding by...
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Currently, I am preparing a single-cell suspension of the dorsal root ganglia of mice. The sample is from one mouse (6 DRGs). The tissue was digested using type II collagenase at 37°C for 30...
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I'm having trouble establishing a DNA index protocol for B lymphocytes because, after the fixation step, there is a significant loss of anti-CD20 fluorescence. Does anyone have any tips on how to...
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From Classical to Relativistic Mechanics via Maxwell This issue is analyzed in context of the Electromagnetic Mechanics project: INDEX -Electromagnetic Mechanics (The 3-Spaces Model)
03 September 2025 6,156 0 View
Dear All! I would like to ask help for a protocol I am currently trying to establish. I am using a protocol (see attached word doc) based on the works of Quinet et al. 2017. The fibers look like...
02 September 2025 10,024 0 View
I am currently preparing to administer Sorafenib to model mice weighing approximately 20 g, with a dosage of 30 mg/kg in a total volume of 200 μL via gavage. Could anyone provide guidance on the...
19 August 2025 6,786 1 View
I'm planning to culture lymphocytes isolated from the whole blood. I know that both media are efficient in isolating PBMCs, but I'm wondering which one I should use to isolate PBMCs for...
13 August 2025 3,042 2 View
Hello, I am interested in conducting an experiment using TMS over motor cortex. But, I cannot estimate some participants' motor threshold for a reason that I cannot explain here. In this case,...
12 August 2025 8,769 0 View
I’m planning an in vivo study on the radioprotective effects of resveratrol in adult male C57BL/6 mice via daily oral gavage. In parallel, I will gavage a specific live strain of Lactobacillus...
09 August 2025 5,092 1 View
Dysphagia after fundoplication: are we blaming a tight wrap only? Waht about a tight cruroraphy?
08 August 2025 6,677 0 View
Anyone can tell me how to improve the transfection effeciency in old T cells that have been cultured for long time. I tried to transfect human PBMC derived T cells which have been cultured more...
07 August 2025 4,273 0 View
Dear colleagues, We invite you to submit your work to the 17th ACM International Conference on Future Energy Systems (ACM e-Energy 2026), the premier venue for research at the intersection of...
06 August 2025 4,970 2 View
Which book on dance has been translated to a dozen languages?
06 August 2025 235 1 View
I'm planning to detect lipid-related signals on paraffin-embedded tissue sections and have the BODIPY 558/568 C12 lipid probe. I've found that it's commonly used for lipid droplet labeling in live...
01 August 2025 7,873 1 View
I am working on research project which is based on "therapeutic potential of plant extract on d galactose induced premature aging in mice" in which I am inducing disease by giving d gal...
29 July 2025 3,638 1 View
I am currently working on a project involving antibody-drug conjugates (ADCs) and encountered an issue with the adsorption efficiency of Protein A/G magnetic beads. After I injected mice with ADC,...
24 July 2025 6,689 1 View
I’m using the GigaPack III XL packaging extract kit (Agilent) for phage packaging. In the control, I’m getting a bacterial lawn but no plaque formation. I used the wild-type control DNA and the...
22 July 2025 4,227 0 View
I put fe2o3, h3po4, and water in the reactor to make FePO4-2H2O (dehydrate) and react at 70~100℃ for 24 hours. (metal/P = 0.97~0.99) Then I measured the pH of the solution that was made, and it...
22 July 2025 5,711 0 View
I prepared a culture media of L-15 with 20% FCS. After culturing cell for 8 days and feeding I saw these structures. But I'm not understanding this. Wether it can be cell or contamination. I am a...
20 July 2025 6,275 0 View
I'm working for a year with a murine HSC line, named FDCP-Mix. It's pain in the a** for starting in culture! Every time I want to start a new culture I spend a lot of vials and time. I follow the...
17 July 2025 3,724 0 View
This compound is a NEDD4-1 inhibitor with demonstrated anticancer activity. Literature indicates it specifically suppresses the proliferation of human melanoma cells, which is highly relevant to...
16 July 2025 7,571 2 View