Hi everyone! I am working with bacteria and fungal collected from marine sediments. I would to know the methodology since the preparation of biomass until the extraction and stored of secondary metabolites,
Thanks!
Marine sediment samples are collected, and bacteria and fungi are isolated using standard microbiological techniques. The isolated strains are cultured in appropriate liquid media, and the biomass is harvested, washed, and freeze-dried. The dried biomass is then extracted using organic solvents like ethyl acetate or methanol, with sonication and overnight incubation. The crude extracts are concentrated under vacuum, transferred to pre-weighed vials, and the solvent is completely evaporated. The extracted secondary metabolites are weighed and stored at -20°C or -80°C for further analysis and characterization.
Nice answer by Abdelhak Maghchiche but do keep in mind that many parameters can alter your results: growth media, growth time (log/stat growth), stress on the bacteria/fungi, and finally the extraction solvent and extraction conditions you use.
For example, see:
https://www.mdpi.com/2079-6382/9/2/48
If you search "which solvent to extract for antimicrobial" you will find many more
The extraction of secondary metabolites from marine sediment-derived bacteria and fungi involves culturing the isolates in appropriate media, harvesting the biomass, and extracting the compounds using organic solvents like ethyl acetate or methanol, with parameters such as growth media composition, growth phase, stress conditions, extraction solvent choice, and extraction conditions significantly impacting the yield and profile of the extracted metabolites, highlighting the importance of optimizing these factors to maximize the recovery of high-quality secondary metabolites for further analysis and characterization.
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