The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
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Questions related to Brain
I have a vertebrate tissue sample and i need to seperate each enzyme present in the sample by performing PAGE I need your suggestion in this regard, which gel run should i perform ? Is it NATIVE...
10 June 2022 4,254 0 View
My data analyst has used a concept called "pre-hypothesis"; it isn't equivalent to the hypothesis equation, but it is for checking Durbin Watson. My problem is that I haven't found any source...
07 June 2022 6,529 4 View
The majority of short chain fatty acids (SCFA) is synthesized in the large intestine by gut microbiota but I was wondering how short chain fatty acids are synthesized in neurons and in the brain.
07 June 2022 197 0 View
Hello, I have 100 tissue samples (50 cortex and 50 hippocampus) that have been homogenized (sigma RIPA + sigma protease inhibitor (PI)). I have made three supernatant aliquots per sample. I...
06 June 2022 8,085 0 View
Depression is a mood disorder that involves a persistent feeling of sadness and loss of interest. What are the causes, effects and probable solutions to depression? Sharing is caring. Thanks!!!
02 June 2022 1,292 47 View
Hello, Recently, during the EEG signal recording, a low-frequency fluctuation with a very high amplitude, as shown in the attached video, is added to the EEG signals. It suddenly appears during...
28 May 2022 7,106 9 View
I have done a gold conjugate with polyclonal antibody .The intensity of blot developed for my recombinant antigen is very faint .How can I improve my intensity of blot?
26 May 2022 2,286 1 View
Hi everyone, I am currently testing protocols for applying a FITC-conjugated PET ligand for the mitochondrial protein TSPO on human FFPE brain tissue. I'm not having much luck and I don't know...
25 May 2022 5,735 0 View
I'm using the BBMVEC to monitor the BBB in vitro. The medium that I'm using now is Bovine Brain EC Growth Medium from Cell Application. But my PI said this medium is too expensive and asked me if...
25 May 2022 909 0 View
In the context of DREADDs and optogenetics, I was wondering what happens at the single-cell level when you infect an animal with an AAV of a higher titer. I understand that the rate of infection...
11 May 2022 180 0 View
I currently work with tracheal MSCs and would like to measure the expression of aSMA. I used bAct as housekeeper so far, but would like to use a different one for aSMA, because both proteins have...
30 April 2022 7,498 1 View
I isolated Extracellular Vesicles from cell culture conditioned media, and boiled the EVs for 15 min at 100oC. Will that treatment be expected to degrade the metabolites within the EVs, or am I...
26 April 2022 4,460 1 View
I grew up and induced my cells with IPTG for protein expression (GST tagged) the day before yesterday and moved them to 20C shaker. I had a migraine yesterday and could not go in to spin them down...
15 April 2022 8,735 0 View
I would like to have information regarding the RNase MRP function especially in plants and alga, besides, the methods that are used to determine a pre-rRNA processing ( if cleavage occures or...
31 March 2022 5,942 5 View
Dear All As we know, to determine the detection limit for AAS /ICP, we can do 10 blank tests and then calculate the standard deviation, so that LOD and LOQ can be calculated. My question is how...
24 March 2022 9,087 4 View
I work on investigating the microbiome of the grapevines Rhizosphere using Shotgun-metagenome sequencing-Illumina. DNA was extracted using PowerSoil® DNA Isolation Kit - QIAGEN and further...
21 March 2022 2,918 2 View
Hi everyone! I left my mouse brain tissue in the fridge for just over a month without sodium azide and now some of them are stuck together ! I realize azide was necessary but I didn’t realize they...
20 March 2022 7,974 0 View
I have some technical problems with RT-PCR and Western blot. For RT-PCR, I followed the protocol from nature protocol(The isolation and molecular characterization of cerebral micro-vessels) to...
15 March 2022 6,721 0 View
Generally, Covalent inhibitors have a strong binding affinity compared to reversible inhibitors. So what is the ki/ic50/kd/kinact/ki cut-off to define a covalent inhibitor activity?
15 March 2022 4,248 2 View
I have some problems in IF staining on formalin fixed, paraffin embedded mouse brain tumor tissue. I tried a protocol that we have in the lab but it seems not works well.. I don't have any signal...
09 March 2022 9,333 6 View
We are currently producing AAVs in our lab, and have so far used the crude lysate to transduce our neurons in organotypic slice cultures. However, we'll now also need to purify and concentrate to...
04 March 2022 1,023 0 View
I have never worked with brain very much and would love some insight on how to prepare it before IF and a protocol to start from. Thank you much.
03 March 2022 1,130 2 View
I've taken fluorescence microscopy images with 4 channels (DAPI, FITC, Cy3, and Cy5 - Cy5 pseudocolored white) and now I can't figure out how to separate them for analysis on ImageJ or Photoshop....
02 March 2022 4,681 2 View
Dear RG members, I am working on finding the links between joint kinematic and EMG time series in pathological gait cycles. The objective is to try to explain altered kinematics from altered...
28 February 2022 2,316 3 View