The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
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Questions related to Brain
I've read an article about quantifying collagen/fibers decomposition in blood vessels, especially in the media/intima of veins and they used angle orientation/anisotropy index. I want to ask which...
26 February 2022 7,288 0 View
I recently received some whole mouse brains from a lab. The brains were in 4%PFA for almost 2 years. I took one of the brains out, washed it in PBS and then submerged it in 30% sucrose (in PBS)...
25 February 2022 8,221 2 View
Hi all, I am conducting 10 minute gap free voltage clamp recordings in hippocampal slice cultures, and would like to be able to monitor the Cm, Rm and Ra at regular intervals (possibly every two...
23 February 2022 1,870 5 View
Dear colleagues- we routinely record local field potentials in freely moving mice from hippocampal CA1 and prefrontal cortex with ~ 125um depth electrodes, but would like to record multi neuronal...
23 February 2022 3,405 2 View
In systems analysis, wavelet transform is used compared to Fourier transform, because wavelets are functions in which most of their energy is concentrated over a short period of time and converges...
18 February 2022 1,865 4 View
We have antibiotic resistance genes cloned into the pET(+)-21 vector and transformed in DE3 E. coli cells. I realize that this system is used for recombinant expression of proteins, however we...
16 February 2022 7,741 3 View
Hello, I am working on the inselico study of different compounds and i am looking for a software which can tell me about the inhibition or activation of a particular pathway by a compound. Please...
13 February 2022 6,375 1 View
i am working on biosafety testing of transgenic algae, I want to know what is recommended time duration for testing the substance on adult zebrafish. I know for zebrafish embryo toxicity test it...
12 February 2022 1,268 0 View
I am looking for a good intracardiac mouse perfusion that is suit well for spinal cord. I've already done a lot of intracardiac perfusion on adult mice to do IHC on brain. Now I work on spinal...
11 February 2022 8,700 0 View
I have doubts about which is the best and most suitable amplifier. We own the AxoClamp 2B and the Axopatch 200B. Thanks a lot for the help.
10 February 2022 4,645 4 View
culture morphology
08 February 2022 7,686 3 View
Would a mouse spleen be expected to have more CD4 or CD19 cells? References to literature or explanations are greatly appreciated.
04 February 2022 1,546 1 View
What is the difference between the human cerebellum and mouse cerebellum? Can the results obtained in the analysis of the mouse cerebellum be extrapolated to the human cerebellum?
02 February 2022 7,023 6 View
I am trying to dual IHC stain both a cytoplasmic marker (CK19) and a nuclear marker (Ki67 or CC3). Individually, these stains look beautiful and even during the first stain of the dual stain...
01 February 2022 1,755 1 View
I'm modeling the habitat suitability of a species. I know that you can project the model to future scenarios when setting up the modeling, but can you project your maxent model to future scenarios...
31 January 2022 3,465 2 View
There is a field of regenerative medicine to treat strokes and TBI, which involves activation of endogenous neural stem cells or grafting various stem cell types into a brain, developing them to...
29 January 2022 4,904 0 View
To understand how glucose is being metabolized in our cell conditions, 13C Glucose was used as a tracer. To determine the carbon contribution from tracer glucose in each metabolite, the MPE was...
21 January 2022 6,520 1 View
Hi all, I have stained my tissue section (cry-section) with DAPI but I am unable to observe stained nucleus in my sample. I have done a few times of imaging on my sample (I can still see the...
20 January 2022 419 1 View
I work on a time series forecasting problem. So I need to learn the best algorithm that performs well in time series forecasting. Please help me.
13 January 2022 2,351 19 View
I would like to do an IF of alpha1-syntrophin on PE brain tissue but I detect no signal. I already tried 2 different ways to proceed antigen retrieval (AR): heat in citrate buffer at 95°C or...
13 January 2022 3,915 0 View
Hi, I am performing gene ontology by using DAVID online software. I have submitted the upregulated genes to look at the biological process, molecular function, and KEGG. I am looking at the role...
13 January 2022 4,755 0 View
I have a bunch of precious samples that appear underfixed for immunofluorescence with a particular antibody. They are all in frozen OCT blocks now. Should I melt them down and re-fix them or can I...
12 January 2022 1,529 6 View
Most of the 3D printing resins are synthetic chemicals. Is there any biopolymers to replace resins. If so kindly share some lietrature and suggestion for using biopolymers for 3D printing
10 January 2022 2,379 4 View
Hi everyone, I want to fix osteoarthritic chondrocytes in 6-well plate with paraformaldehyde. Before fixation, it takes at least three days for the chondrocytes to adhere to the surface of the...
27 December 2021 9,272 4 View