The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
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Questions related to Brain
How do we check the working of designed primers of micro RNA? I have designed primers for my miRNA sequences. I ran normal PCR to check the working of primers, but I couldn't see any bands from my...
02 February 2024 785 7 View
An error message is " Simulation takes longer time" and "Check your units and frequency", However, antenna dimensions at Mhz. Kindly give your valuable suggestions
01 February 2024 6,253 2 View
I'm going to use the RIPA buffer, but I don't know if I should use the sonicator or polytron homogenizer
31 January 2024 7,390 1 View
Greetings, I am a graduate student interested in research involving the measurement of ultrasonic vocalizations in mice. An essential component to potential experiments of mine is the use of...
31 January 2024 9,168 0 View
I prefer low budget options. I will appreciate if you can share your experience with such devices: strengths, weaknesses, etc. I will use them in small creeks and reservoirs. Thank you in advance.
31 January 2024 8,890 0 View
We have been trying to produce lentivirus with the Tet inducible system for a while, but we are getting no signal in the infected cells after infection. However, after transfection, there is a...
31 January 2024 9,732 3 View
Researchers working on computer models for predicting toxicity in non-target organisms
30 January 2024 2,706 3 View
It's extremely weird. In my current research, my gene X is essential for cell survive. I knockout gene X in the cells (CreERT2; X flox/flox) by treating 1uM 4-OHT for 24h. So X will depleted very...
30 January 2024 3,046 0 View
I am trying to embed generated organoid onto a matrigel and when I try following parafilm embedding methods and i cant move matrigel off the parafilm.
29 January 2024 9,600 0 View
Hi all, I recently tried conducting ChIP-qPCR assay, however, the negative regions (white/gray) both showed high enrichment with the Flag antibody(IP'ed group) rather than with the control...
27 January 2024 1,410 0 View
Recently, we submit a work on carbon dot-based electronic device to a journal. In the work, ultraviolet photoelectron spectroscopy (UPS) measurement was applied to determine the valence band...
27 January 2024 8,244 3 View
What and how?
26 January 2024 8,979 0 View
How to sterilize a polymer grafted with a photoinitiator before cell culture?
24 January 2024 9,519 2 View
Techniques Tools required Step by step Procedure
22 January 2024 8,936 0 View
I am using Qiagen Blood RNA Mini kit to isolate RNA from whole blood.. and the A260/A230 is quite low, even below 1. My main downstream application is RNA-seq. and I am not sure if this low ratio...
18 January 2024 9,894 0 View
I have revived an adherent cell line from second day I noticed a whitish cloudy layer in media. When i disturb the media the cloudy layer get easily dispersed into the media (more no. of dead...
17 January 2024 7,668 4 View
I have access to quite an old Hitachi fluorescence spectrophotometer. Unfortunately the requisite software is missing. Would anyone be able to provide me with copy of the FL solutions...
16 January 2024 3,346 0 View
I'm running XF T Cell persistence assay in a mini Seahorse. I used to not have problems until I start getting some negatives values in OCR measurements, and also errors with both background wells,...
13 January 2024 4,434 0 View
Toxicity evaluation of pharmaceutical compounds in an aqueous medium is essential. ECOSAR software predicts the toxicity of compounds can any one please give the details about it?
05 January 2024 4,915 3 View
What is the role of microorganisms in composting and humus formation and role of microorganisms in nutrient cycling in the carbon cycle?
03 January 2024 7,326 3 View
What might a sci-fi narrative look like from the perspective of elites justifying leaving the masses behind to secure their own survival? How might it portray their moral reasoning and ethical...
30 December 2023 6,789 1 View
I'm trying to study spatial transcriptomics by myself, but it's not easy to understand. So, spatial GEX is to cut the tissue into slides, attach it to the slide where the probes are located, and...
25 December 2023 6,048 1 View
I concatenated four sequences. Can I find the best model for the concatenated sequences and then run the maximum likelihood tree with the best model in MEGA X?
15 December 2023 7,467 0 View
In summary, since intercourse has been defined as the basic form of sexuality, and the only natural, healthy, and moral form of physical contact, it has automatically been assumed that this is...
15 December 2023 4,786 0 View