Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Can ethanol be used as an analgesic?

If ethanol has analgesic properties, why isn't it used for clinical purposes?

18 February 2021 8,227 4 View

How to decrease the time for publication of paper in a journal ?

It takes a lot of time in publishing the paper in the journal, from few months to a year so how one can able to decrease the time for publication.

17 February 2021 2,997 14 View

Forced Vibration Testing using FDD?

Hi, Can we use FDD algorithm for forced vibration response (sinusoidal response produced from eccentric mass vibrator)? Thanks

22 January 2021 5,851 2 View

Any suggestion on self-efficacy scale ?

Dear researchers, I am planning on conducting a research on the relationship between Language learning strategies, by Oxford (1990) and self-efficacy. I have found Morgan-Jinks Student Efficacy...

22 January 2021 8,432 3 View

I need to apply a questionnaire from an article. What statistical analysis do I need to use?

Hello, I found a survey about attitude toward language in an article which I find fit to use it in my thesis. The survey has 30 questions, which were put into 5 factors through factor analysis...

21 January 2021 9,419 3 View

What should my objective ?

I am going to start my master program (msc agri hons) research in rull of potassium in tomato vigetable crop so what should my objective please guide me Thanks

13 January 2021 4,635 6 View

Why did I obtain abnormal staining by TUNEL assay?

I use Abcam TUNEL Assay Kit - HRP-DAB (ab206386) on mouse tissues. The staining showed a pattern that I expected to see with a specific layer of cells dying due to drug treatment. But the...

12 January 2021 144 1 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View

I'm using AMOS for SEM--Why does my chi-square value change when I specify groups?

My study involves 2 groups (Black and Latinx participants, N=816). I've run my structural equation models with the data all together and will get a completely different chi-square when I specify...

22 February 2021 9,342 4 View

Are negative valued indicators allowed in SEM?

Hello, Using secondary and continuous data, i am trying to run a SEM with two latents. One indicator has both negative and positive values. Is it wrong? (I am gonna run the model in AMOS) Thx on...

14 February 2021 2,801 8 View

Moderation test of reflective construct in AMOS?

Hi dear researchers, I need your guidance on the moderation test of a reflective construct (i.e., Uncertainty avoidance with three items) with high and low UA. I run the moderation test with the...

08 February 2021 370 7 View

How to obtain confidence intervals in Amos?

Dear all, I would like to know your opinion on the following issue I am struggling with. I am estimating a multigroup model with several factors all correlated among them (they represent...

04 February 2021 1,381 4 View

AMOS - Question regarding correlated errors in CFA, what next?

Hi all, Hopefully this is just a quick question for those of you au fait with CFA in AMOS. I am brand new to this. I'm looking at model (small sample size, yes I know) with 5 latent variables....

26 January 2021 4,425 2 View

Is it possible to implement a Latent Profile Analysis with AMOS?

Could you kindly recommend me any useful totorials to learn how this statistical anaysis should be applied? Thanks

10 January 2021 3,633 2 View

Can we use control variables during Structural Equation Modeling with Amos?

We want to explore a mediating relationship employing SEM with Amos SPSS. Our independent variable is attitude toward wife abuse. mediator is exposure to male marital violence, and outcome...

07 January 2021 4,481 3 View