Can anyone recommend polyclonal or monoclonal antibodies for
Tet methylcytosine dioxygenase 2 (TET2)
Tet methylcytosine dioxygenase 3 (TET3)
DNA methyltransferase 3 beta (DNMT3B)
Ideally, they should work on bovine tissues using immunohistochemistry methods on paraffin sections and western blots. We are interested in testing the corpus luteum of a cow. However, it is a tissue that contains some fat, which can hinder antibody binding. We have already tried several antibodies, and none of them worked. However, gene expression studies of TET2, TET3, and DNMT3B revealed their expression in this tissue.
Not a personal recommendation, but GeneTex and Biorbyt have anti-TET3 pAbs that they say are bovine reactive:
https://www.genetex.com/Product/Detail/TET3-antibody-C3-C-term/GTX121453
https://www.biorbyt.com/tet3-antibody-orb1418875.html
and Millipore-Sigma has bovine reactive anti-DNMT3B:
https://www.sigmaaldrich.com/US/en/product/sigma/sab2108295
- Badges
- Science topic