The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
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Questions related to Brain
When I look up studies about tdcs, it is always assumed that the electrical current produces polarisation only at the electrode surface. I have never been able to find any studies who clarify...
20 March 2021 5,865 3 View
I am doing some western blotting via SDS PAGE with human brain tissue. I am using whole human sera as my primary antibody because its from a rare disorder, and plan on using mouse anti-human IgG...
18 March 2021 5,118 1 View
Hi, I am performing gelatin zymography to see MMP 2 and 9 expression. I am having several technical difficulties. I am using conditioned media from 3T3-L1 preadipocyte and MDA-MB 231 cells. I am...
18 March 2021 9,309 3 View
I am having problems obtaining insulin stimulated glucose uptake in differentiated 3T3-L1 adipocytes. I have tried several differentiation protocols but I only get a 30% increase in glucose uptake...
18 March 2021 6,853 1 View
Hi All, I’m pretty new to cryosection so any advice or suggestions are greatly appreciate. The brain tissues are fixed in 4% PFA and cryoprotected in 30% sucrose until they sink. Then samples...
07 March 2021 9,268 2 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
02 March 2021 1,127 2 View
I've been troubleshooting my IF ERα protocol and I'm curious to see what reagents other people use in this stain. Thanks.
01 March 2021 4,525 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
28 February 2021 645 6 View
Dear friends and colleagues. Does anyone have any opinions, or know of any studies, regarding the best material (& placement) to use for in vivo electrophyisological Studies? In particular,...
27 February 2021 8,635 0 View
Hi sir, Is there any possibility to use any reinforcement algorithms for feature selection procedure. Or will it be used for prediction process? Since it is based on action and reward based...
24 February 2021 2,830 7 View
Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...
19 February 2021 1,757 1 View
I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.
10 February 2021 1,525 5 View
I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...
10 February 2021 2,837 3 View
What are temporal limits in determining brain metastability? In other words, how long does the minimum length of the scan segment using fMRI have to be for the result to be of any value? I'm...
08 February 2021 2,538 2 View
Hello! We have a project where participants engaged in reading, thinking and then responding to an ethical dilemma. We used the EMOTIV 14 channel headset to track brain activity. The reading,...
30 January 2021 4,290 3 View
hello I am working on a project that includes gene delivery to the brain. my intentions are encapsulating a gene in a Chitosan NP and IV administration the NP to rats. beside plasmids is there...
30 January 2021 4,549 5 View
Hi people, I am studying bat brains through endocranial casts and I cannot identify which structure could be present in sort of a canal located dorsally to the cribriform plate. It is like...
28 January 2021 978 6 View
According to the vertebrate literature, I got the impression that head-direction cells are always anchored to an allocentric reference frame. This can easily be tested with freely moving animals....
28 January 2021 3,976 3 View
Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...
27 January 2021 1,306 8 View
I'm staining some brains with several stemness markers but the OCT4 antibody that I have been using is not working. I would really appreciate your recommendations! Thank you!
26 January 2021 3,320 3 View
Dear colleagues, We transplant human cells in the mouse brain. After perfusion with 4% PFA (+ 4-6 hours post-fix) we want to identify histologically the human cells in a brain section. We have...
20 January 2021 485 3 View
I'm looking to do some IHC/IF staining of X. laevis brains that have been frozen in OCT media for cryosectioning. I understand how to do the cryosectioning, but I am hoping for help/protocols that...
15 January 2021 9,893 2 View
Is there a typical range in the power spectrum that each person has in the individual brain areas (19 electrodes, 10-20 system) and frequency bands (theta, alpha, beta gamma) during a rest...
14 January 2021 5,770 3 View
Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...
14 January 2021 9,598 0 View