The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
7,977 views 7,177 posts
Questions related to Brain
I need to study interaction between a metal ion and a multiatomic compound using DFT.
01 June 2024 9,576 4 View
TEM imaging
31 May 2024 3,851 2 View
Hi to everyone, We are working with 3T3-L1 pre-adipocytes and having some potential contamination that I don´t have ever seen. As you can see in both pictures (10X and 40X), there are a lot of...
31 May 2024 1,615 0 View
Does anybody know whether CFSE is retained in cell nuclei after treatment of cells with pepsin solution in HCl?
30 May 2024 1,402 0 View
Hello, We recently seeded a HeLa cell line purchased from ATCC, which was received over six months ago (stored at LN2). We used the following media composition: DMEM supplemented with 10% FBS, 1%...
28 May 2024 7,351 2 View
I have synthesized CaFeO3 three times using metal nitrates through sol-gel method but each time XRD analysis show that its peaks match with Ca2Fe2O5. Metal to acid ratio is 1:1.38. I've also tried...
26 May 2024 3,410 1 View
Bescause this is my first time to use this web so if there is any mistake i hope u guys can forgive me. Thank you so much!
26 May 2024 4,768 0 View
I've coated plastic water bottles (PET) with Nile Red to detect the plastic degradation by Ideonella sakaiensis. When using the settings for Sypro Ruby, the coated and uncoated plastic looks the...
24 May 2024 3,446 0 View
Hi, I have been working with 3T3-L1 cells for over a year now.However, over the last couple of months they stopped differentiating into adipocytes. I seed at 200k cells/well (12 well plate), and...
24 May 2024 3,400 1 View
I have recently started working with heterochromatin . I am trying to quantify the H3K9Me levels but i am failing to do so. Sometimes i see faint duplex band . I do TCA Lysis and then follow up...
23 May 2024 2,115 2 View
I need to do immunohistochemistry in mouse brain, particularly in neurotensin-cre mouse line.
23 May 2024 3,620 7 View
I have obtained 100 ul of pre-amplified cDNA using the SSIV SINGLE CELL PRE-AMP KIT. Now i want to make a dilution because my samples are limited and i cannot directly use the cDNA. So what...
23 May 2024 6,062 3 View
I purified my recombinant GST-tagged protein in Tris buffer at pH 7.6 (the protein’s PI is 7.11) using a 50 mM reduced glutathione concentration in the elution buffer. However, after dialysis to...
22 May 2024 10,017 2 View
Hi all, . I would be grateful if you could help me with identifying any way to stimulate a stress response or inflammatory response in Chinese Hamster Ovarian Cells. Do they have a normal...
21 May 2024 4,965 0 View
More and more relationships are collapsing in this 21st century due to the fact that women are becoming more and more romantic. They struggle for freedom from the past world dominated by men. In...
20 May 2024 5,717 1 View
how do I answer this question/ refine and make it more specific
19 May 2024 7,210 0 View
How do the elasticity and temperature of a medium affect sound propagation and elasticity density and temperature affect the speed of sound?
18 May 2024 2,853 0 View
Hi everyone, I try to do a simulation about heat transfer of supercritical hydrogen in a cooling channel. Properties of H2 is obtained from NIST and displayed by an UDF function. I used the k-w...
17 May 2024 4,141 2 View
I have cloned a 6.46 kb having restriction sites for enzymes BamHI and Not I in pcDNA3.1 vector. After colony pcr screening, the result shows positive result with primer specific to vector and...
16 May 2024 197 4 View
I am planning to do invitro transcription and capping for an mRNA vaccine generation. Can anyone suggest whether it should be done on a bench top or inside a laminar hood?
15 May 2024 267 3 View
I was wondering if you can move immunofluorescence ready sample that is in the form of suspension on 96-well plate to another well plate. Is that possible?
15 May 2024 634 1 View
Do microorganisms release nutrients in soil and role of microorganisms in recycling carbon present in this food chain?
14 May 2024 2,048 2 View
Why are microorganisms important in recycling carbon in the environment and roles of microorganisms in waste water recycling?
14 May 2024 7,717 2 View
I want to read about studies or experiments who proved that being brave and persistent are suceeding factors. Are there any new aspects or findings e.g. from neuroscience?
13 May 2024 4,658 3 View