The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of... | Contact experts in Brain to get answers
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Hello, everyone! It is known that DNA methylation in promoter regions containing CpG islands is responsible for downregulation of genes. How about genes which don´t contain CpG islands in their...
29 April 2021 3,693 3 View
Hi! I am looking for a fluorescente probe to evaluate ROS levels in specific subregions of mouse brain. I tested DHE but the staining was totally unspecific!!! To test DHE before the...
20 April 2021 6,708 3 View
Severe COVID-19 infection leads to respiratory complications, which may adversely affect the supply of oxygen to the brain. Due to insufficient supply of oxygen, the central nervous system may get...
19 April 2021 1,024 3 View
From the functions that the hemispheres of the brain does,Do you think these functions or tasks (writing , reading arithmetic reasoning) are further segmented into different parts of the left...
19 April 2021 3,913 1 View
Current research topic is - Fdi determinants, where there are FDI inflow is the dependent variable and real rate of exchange, inflation, Real gdp and growth of real gdp are the independent...
19 April 2021 1,276 20 View
I am trying to make a thick cut of mouse brain slices but having trouble with compressing the brain while cutting. I used a typical metal razor blade. I saw some people suggest sapphire or ceramic...
15 April 2021 7,484 2 View
I want to measure level of serotonin, BDNF and IL-6 from SD rat brain, but there is no detailed protocol about what to do with the brain part to be able to measure by ELISA. Please help me. Thank you
15 April 2021 1,568 5 View
Hi, I want to stain brain sections of mice which have been xenografts with tumor and treated with a new anti cancer agent. Can some please share the protocol with me including tips and does and...
12 April 2021 6,636 2 View
Hi everyone, I am looking for a good fluorescent AAV1-SYN to trace axons from the brain to the spinal cord. It will be co-injected with another AAV1-SYN-eGFP, so -ideally- it should be red. Our...
12 April 2021 5,041 2 View
I work with infarcted brain tissue and I am checking the MMPs expression. The thing is that the gels are kind of dirty (specially around the top of the gel), and I would really appreciate if...
12 April 2021 3,439 3 View
I am about to run mice brain samples in the HPLC. I have optimised the standards however some methods are spiking their samples. Why do I need to do this?
12 April 2021 1,095 4 View
Hi, I am reading some papers that use GLM to deal with repeated measure. They often mentioned that they used "GLM repeated measures ANOVA". There are also F values and p values reported. For...
10 April 2021 5,249 9 View
MRI, fMRI, EEG etc..
08 April 2021 3,497 3 View
I have tried changing the antiroll distance, clearance angles, tried temperature values of -20 to -15. Sometimes just a couple of sections come at -17. But many a times not. Recently made an...
08 April 2021 1,215 2 View
The cryostats in our lab is not delivering sections as expected. it seems like whatever section thickness we choose (e.g. 20, 30, 40, 60 microns), the real thickness we get are around 20 microns...
07 April 2021 206 2 View
Hi everyone, We are going to perform some immunohistochemistry using c-fos antibody to look at the expression patterns of fos protein in different brain regions. I know that most people...
06 April 2021 2,464 1 View
When doing IHC-F, I found a lot of ugly holes on my mouse brain slices(20um). That's really bad and I don't know how to avoid the damage to my samples. After perfusion fixation, 4% PFA were used...
06 April 2021 892 1 View
I am about to try some ChIP-seq on rat brain tissue. I was wondering if 1 - anyone has a nice protocol that is reliable in their hands and 2 - if it is possible to either flash freeze or fix the...
06 April 2021 6,743 0 View
I'm working on an EEG experiment using the oddball paradigm. My experiment already has 2 within-subject factors. I want to introduce a third factor but I don't want to make the experiment too...
01 April 2021 4,357 3 View
I've constructed several AAV vectors with TBG-promoter (which is liver specific) and FLAG-tag , while several of them cannot express in 293T cells (by Western Blot check with flag-tag antibody)....
01 April 2021 832 2 View
I have brain tissues that are fixated for a few years and I plan on staining them using fluorescent Neu-N antibody. I know that it usually does not work without antigen retrieval. I could not find...
25 March 2021 6,688 3 View
Where can i learn rat dissection for brain tissue biochemical analysis, mainly the experiment room and surgical tools setup
24 March 2021 2,595 2 View
For basic time-intensity-analsis (TTP, MTT, MI, Slope...) on MRI images eg. of the liver, vessels and the lung, I am searching free or open source software for PC or OSX. Beyond these basics,...
23 March 2021 4,619 5 View
Hello everyone, I have some brain slides that I CV stained, and I need to learn how to assess infarct volume and quantify it comparing it to the other hemisphere or my controls. I looked online...
22 March 2021 6,186 2 View